| Title: |
Crystallization and preliminary diffraction analysis of Wzi, a member of the capsule export and assembly pathway in Escherichia coli |
| Authors: |
Bushell, Simon R.; Lou, Hubing; Wallat, Gregor D.; Beis, Konstantinos; Whitfield, Chris; Naismith, James H. |
| Contributors: |
The Wellcome Trust; University of St Andrews.School of Chemistry; University of St Andrews.Biomedical Sciences Research Complex; University of St Andrews.EaSTCHEM |
| Publication Year: |
2011 |
| Collection: |
University of St Andrews: Digital Research Repository |
| Subject Terms: |
Wzi; Polysaccharide capsules; Escherichia coli; Outer-membrane; Klebsiella-pneumoniae; Colanic acid; Protein; Polysaccharide; Expression; Translocation; Biosythesis; Organization; Crystals; QD Chemistry; QD |
| Description: |
Supported by a grant from The Wellcome Trust (081862/Z/06/Z) ; External polysaccharide capsules provide a physical barrier that is employed by many species of bacteria for the purposes of host evasion and persistence. Wzi is a 53 kDa outer membrane beta-barrel protein that is thought to play a role in the attachment of group 1 capsular polysaccharides to the cell surface. The purification and crystallization of an Escherichia coli homologue of Wzi is reported and diffraction data from native and selenomethionine-incorporated protein crystals are presented. Crystals of C-terminally His(6)-tagged Wzi diffracted to 2.8 A resolution. Data processing showed that the crystals belonged to the orthorhombic space group C222, with unit-cell parameters a = 128.8, b = 152.8, c = 94.4 A, alpha = beta = gamma = 90 degrees. A His-tagged selenomethionine-containing variant of Wzi has also been crystallized in the same space group and diffraction data have been recorded to 3.8 A resolution. Data processing shows that the variant crystal has similar unit-cell parameters to the native crystal. ; Peer reviewed |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
English |
| ISBN: |
978-0-00-285064-3; 978-7-86501-463-2; 0-00-285064-8; 7-86501-463-5 |
| Relation: |
Acta Crystallographica. Section F, Structural biology and crystallization communications; 5569882; 000285064800018; 78650146359; https://hdl.handle.net/10023/1800; https://www.scopus.com/pages/publications/78650146359; 081862/Z/06/Z |
| DOI: |
10.1107/S1744309110040546 |
| Availability: |
https://hdl.handle.net/10023/1800; https://doi.org/10.1107/S1744309110040546; https://www.scopus.com/pages/publications/78650146359 |
| Rights: |
This is an open access article distributed under a Creative Commons licence (http://creativecommons.org/licenses/by/2.0/uk/legalcode) which permits you to reuse and copy the article provided the original authors and source are cited. |
| Accession Number: |
edsbas.5EB66A9F |
| Database: |
BASE |