| Title: |
Modulation of a cytoskeletal calpain-like protein induces major transitions in trypanosome morphology |
| Authors: |
Hayes, P; Varga, V; Olego-Fernandez, S; Sunter, J; Ginger, ML; Gull, K |
| Publisher Information: |
Rockefeller University Press |
| Publication Year: |
2016 |
| Collection: |
Oxford University Research Archive (ORA) |
| Description: |
Individual eukaryotic microbes, such as the kinetoplastid parasite Trypanosoma brucei, have a defined size, shape, and form yet transition through life cycle stages, each having a distinct morphology. In questioning the structural processes involved in these transitions, we have identified a large calpain-like protein that contains numerous GM6 repeats (ClpGM6) involved in determining T. brucei cell shape, size, and form. ClpGM6 is a cytoskeletal protein located within the flagellum along the flagellar attachment zone (FAZ). Depletion of ClpGM6 in trypomastigote forms produces cells with long free flagella and a shorter FAZ, accompanied by repositioning of the basal body, the kinetoplast, Golgi, and flagellar pocket, reflecting an epimastigote-like morphology. Hence, major changes in microbial cell form can be achieved by simple modulation of one or a few proteins via coordinated association and positioning of membrane and cytoskeletal components. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
https://doi.org/10.1083/jcb.201312067 |
| DOI: |
10.1083/jcb.201312067 |
| Availability: |
https://doi.org/10.1083/jcb.201312067; https://ora.ox.ac.uk/objects/uuid:8d819346-024d-43bd-9700-1d0dc945f598 |
| Rights: |
info:eu-repo/semantics/openAccess ; CC Attribution-NonCommercial-ShareAlike (CC BY-NC-SA) |
| Accession Number: |
edsbas.6029F33D |
| Database: |
BASE |