| Title: |
Alcian blue staining to visualize intracellular hyaluronic acid-based nanoparticles |
| Authors: |
Repellin, Mathieu; Carton, Flavia; Lollo, Giovanna; Malatesta, Manuela |
| Contributors: |
Carlo Pellicciari, Marco Biggiogera, Manuela Malatesta; Repellin, Mathieu; Carton, Flavia; Lollo, Giovanna; Malatesta, Manuela |
| Publisher Information: |
Humana; New York, NY |
| Publication Year: |
2023 |
| Collection: |
Università degli Studi di Verona: Catalogo dei Prodotti della Ricerca (IRIS) |
| Subject Terms: |
Histochemistry; Hyaluronate; Light microscopy; Nanocarriers; Transmission electron microscopy; Alcian Blue; Coloring Agents; Staining and Labeling; Hyaluronic Acid; Nanoparticles |
| Description: |
Investigating at transmission electron microscopy the intracellular trafficking of hyaluronic acid-based nanoparticles remains a challenge due to their intrinsic weak electron density. Here we describe a simple protocol to stain hyaluronic acid that allows visualization of hyaluronic acid-based nanoparticles inside cells at both light and electron microscopy. By applying the critical-electrolyte-concentration Alcian blue method, these nanoparticles were observed as blue dots at bright-field microscopy or filled with fine electron dense precipitates at transmission electron microscopy. |
| Document Type: |
book part |
| File Description: |
STAMPA |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/isbn/978-1-0716-2675-7; info:eu-repo/semantics/altIdentifier/pmid/36152262; ispartofbook:Histochemistry of Single Molecules: Methods and Protocols; volume:2566; firstpage:313; lastpage:320; numberofpages:8; serie:METHODS IN MOLECULAR BIOLOGY; alleditors:Carlo Pellicciari, Marco Biggiogera, Manuela Malatesta; https://hdl.handle.net/11562/1079707 |
| DOI: |
10.1007/978-1-0716-2675-7_25 |
| Availability: |
https://hdl.handle.net/11562/1079707; https://doi.org/10.1007/978-1-0716-2675-7_25 |
| Accession Number: |
edsbas.6034423C |
| Database: |
BASE |