| Title: |
Separation of chitosan by degree of acetylation using simple free solution capillary electrophoresis |
| Authors: |
Mnatsakanyan, Mariam; Thevarajah, Joel; Roi, Rozet; Lauto, Antonio; Gaborieau, Marianne; Castignolles, Patrice |
| Contributors: |
Australian Centre for Research on Separation Science, School of Science; Western Sydney University; Nanoscale Organisation and Dynamics Group (NANO); Bioelectronics and Neuroscience Research Group (BENS) |
| Source: |
ISSN: 1618-2642. |
| Publisher Information: |
HAL CCSD; Springer Verlag |
| Publication Year: |
2013 |
| Collection: |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
| Subject Terms: |
capillary electrophoresis chitosan degree of acetylation critical conditions NMR spectroscopy; capillary electrophoresis; chitosan; degree of acetylation; critical conditions; NMR spectroscopy; [CHIM]Chemical Sciences; [CHIM.ANAL]Chemical Sciences/Analytical chemistry; [CHIM.MATE]Chemical Sciences/Material chemistry; [CHIM.POLY]Chemical Sciences/Polymers |
| Description: |
International audience ; Chitosan is a biopolymer of increasing significance, as well as a renewable and sustainable material. Its main molecular characteristics are molar mass and degree of acetylation (composition). Precise average degrees of acetylation were measured by quantitative 1 H solutionstate NMR spectroscopy. While number-average degree of acetylation had already been determined by 1 H NMR spectroscopy, weight-average degree of acetylation are also determined and may be more relevant for some properties, such as mechanical properties. We report the first separation of chitosan according to its degree of acetylation using free solution capillary electrophoresis. Capillary electrophoresis separates chitosan in the 'critical conditions': the molar mass plays little role and the separation is by the degree of acetylation. It characterizes the heterogeneity of chitosan samples in terms of composition (dispersity of the distribution of degrees of acetylation). This heterogeneity (broad distribution of degrees of acetylation) cannot be neglected contrary to a common assumption found in the literature. This fast and easy separation will allow establishing structure-property relations. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
hal-04075113; https://hal.science/hal-04075113; https://hal.science/hal-04075113/document; https://hal.science/hal-04075113/file/2013%20Ana%20Bioana%20Chem%20chit%20accepted.pdf |
| DOI: |
10.1007/s00216-013-7126-4 |
| Availability: |
https://hal.science/hal-04075113; https://hal.science/hal-04075113/document; https://hal.science/hal-04075113/file/2013%20Ana%20Bioana%20Chem%20chit%20accepted.pdf; https://doi.org/10.1007/s00216-013-7126-4 |
| Rights: |
info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.62300D6C |
| Database: |
BASE |