| Title: |
Genetic variants of RNASE3 (ECP) and susceptibility to severe malaria in Senegalese population |
| Authors: |
Ndao, Bineta Niakhana; Thiam, Fatou; Thiam, Alassane; Ndiaye, Rokhaya; Dieye, Yakhya; Olaso, Robert; Deleuze, Jean-François; Dieye, Alioune; Diop, Gora; Derbois, Céline; Loucoubar, Cheikh; Mbengue, Babacar |
| Contributors: |
Faculté des Sciences et Techniques (FST); Université Cheikh Anta Diop de Dakar Sénégal (UCAD); Institut Pasteur de Dakar; Pasteur Network (Réseau International des Instituts Pasteur); École Supérieure Polytechnique de Dakar (ESP); Centre National de Recherche en Génomique Humaine (CNRGH); Institut de Biologie François JACOB (JACOB); Direction de Recherche Fondamentale (CEA) (DRF (CEA)); Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)); Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA); Biostatistique, Bio-informatique et Modélisation - Biostatistics, Bioinformatics and Modelling Group Dakar; Pasteur Network (Réseau International des Instituts Pasteur)-Pasteur Network (Réseau International des Instituts Pasteur); Dedonder Clayton 2012 and 2014 (Institut Pasteur Network) |
| Source: |
ISSN: 1475-2875 ; Malaria Journal ; https://cea.hal.science/cea-04485685 ; Malaria Journal, 2018, 17 (1), pp.61. ⟨10.1186/s12936-018-2205-9⟩ ; https://malariajournal.biomedcentral.com/articles/10.1186/s12936-018-2205-9. |
| Publisher Information: |
CCSD; BioMed Central |
| Publication Year: |
2018 |
| Subject Terms: |
Senegal; Polymorphisms; RNase3 (ECP) gene; Susceptibility; Plasmodium falciparum; Severe malaria; [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry; Molecular Biology/Genomics [q-bio.GN]; [SDV.GEN]Life Sciences [q-bio]/Genetics |
| Description: |
International audience ; Background Severe forms of malaria (SM) are an outcome of Plasmodium falciparum infection and can cause death especially in children under 4 years of age. RNASE3 (ECP) has been identified as an inhibitor of Plasmodium parasites growth in vitro, and genetic analysis in hospitalized Ghanaian subjects has revealed the RNASE3 +371G/C (rs2073342) polymorphism as a susceptibility factor for cerebral malaria. The +371 C allele results in an Arg/Thr mutation that abolishes the cytotoxic activity of the ECP protein. The present study aims to investigate RNASE3 gene polymorphisms and their putative link to severe malaria in a malaria cohort from Senegal. Methods/results Patients enrolled from hospitals were classified as having either uncomplicated (UM) or severe malaria (SM). The analysis of the RNASE3 gene polymorphisms was performed in 241 subjects: 178 falciparum infected (96 SM, 82 UM) and 63 non-infected subjects as population control group (CTR). Six frequent SNPs (MAF > 3%) were identified, and one SNP was associated with malaria severity by performing a logistic regression analysis SM vs.UM: RNASE3 +499G/C (rs2233860) under age, sex as covariates and HbS/HbC polymorphisms adjustment (p = 0.003, OR 0.43, CI 95% 0.20–0.92). The polymorphisms: +371G/C (rs2073342), +499G/C (rs2233860) and +577A/T (rs8019343) defined a haplotype risk (G-G-T) for malaria severity (Fisher exact test, p = 0.03) (OR 4.1, IC 95% (1.1–14.9). Conclusion In addition to the previously described association of +371G/C polymorphism in Ghanaians cohort, the RNASE3 +499G/C polymorphism was associated with susceptibility to SM in a Senegalese population. The haplotype +371G/+499G/+577T defined by RNASE3 polymorphisms was associated with severity. The genetic association identified independently in the Senegalese population provide additional evidence of a role of RNASE3 (ECP) in malaria severity. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1186/s12936-018-2205-9 |
| Availability: |
https://cea.hal.science/cea-04485685; https://cea.hal.science/cea-04485685v1/document; https://cea.hal.science/cea-04485685v1/file/s12936-018-2205-9.pdf; https://doi.org/10.1186/s12936-018-2205-9 |
| Rights: |
info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.6260D55C |
| Database: |
BASE |