| Title: |
Establishment of an Efficient In vitro Regeneration and Micropropagation Systems of Cassava (Manihot esculenta Crantz) |
| Authors: |
Azad, Md Abu Sayem; Aktar , Mst Mahbuba; Islam, S M Shahinul |
| Source: |
Plant Tissue Culture and Biotechnology; Vol. 35 No. 2 (2025); 285-295 ; 1818-8745 ; 1817-3721 |
| Publisher Information: |
Bangladesh Association for Plant Tissue Culture and Biotechnology (BAPTC&B) |
| Publication Year: |
2026 |
| Collection: |
Bangladesh Journals Online (BanglaJOL) |
| Subject Terms: |
Cassava; Indirect regeneration; Nodal segments; Acclimatization |
| Time: |
Bangladesh |
| Description: |
An in vitro micropropagation protocol for cassava Manihot esculenta was developed through callus culture. The present investigation the highest callus induction was achieved on MS medium containing 8.0 mg/l 2,4-D. Inter node and leaf derived calli were transferred to MS medium supplemented with BAP and NAA for shoot regeneration. In this case 10.0 mg/l BAP alone produced highest number of shoots (4.8 ± 0.3) per explant having average length of 1.72 ± 0.2 cm. The combination of 10.0 mg/l BAP and 0.5 mg/l NAA further enhanced shoot proliferation, producing an average of 5.6 ± 0.6 shoots per explant with a mean shoot length of 3.6 ± 0.2 cm with a regeneration rate of 87%. Moderate BAP concentrations (4.0-10.0 mg/l) were optimal for indirect shoot regeneration, whereas NAA or Kn alone exhibited limited effects. Root induction was most effective on half-strength MS medium supplemented with 2.0 mg/l IBA, which resulted in 100% rooting, with the highest mean root number of 4.8 and root length of 2.8 cm. The regenerated plantlets were successfully acclimatized in pots containing a 1 : 1 mixture of cocopeat and autoclaved soil, exhibiting high survival rates under shade house conditions. Plant Tissue Cult. & Biotech. 35(2): 285-295, 2025 (December) |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
English |
| Relation: |
https://banglajol.info/index.php/PTCB/article/view/86596/56373; https://banglajol.info/index.php/PTCB/article/view/86596 |
| Availability: |
https://banglajol.info/index.php/PTCB/article/view/86596 |
| Rights: |
Copyright (c) 2025 Plant Tissue Culture and Biotechnology |
| Accession Number: |
edsbas.6361C76E |
| Database: |
BASE |