| Title: |
Strategies of investigating interactions between chicken caecum and Salmonella Enteritidis with ex-vivo models |
| Authors: |
Barilleau Augis, Emilie; Sutton, Kate M.; Holbert, Sébastien; Joury, Léa; Pichon, Julien; Velge, Philippe; Guabiraba, Rodrigo; Virlogeux-Payant, Isabelle; Lalmanach, Anne-Christine |
| Contributors: |
Infectiologie et Santé Publique (ISP); Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE); The Roslin Institute; Biotechnology and Biological Sciences Research Council (BBSRC); INRAE Institut Carnot F2E sur Projet “CENTIM” (Coord. AC Lalmanach 2023-2025); ADISSEO “GutMiVir” project (Coord. R Guabiraba, 2022-2025); Emilie Allard-Vannier; Anne Di-Tommaso; Céline Ducournau; Jean-Michel Escoffre; Maxime Gueguinou; Laurie Lajoie; Frédéric Laumonnier; Dévina Ung |
| Source: |
Second Thematic Workshop on 3D Cell Models in Loire Valley ; https://hal.inrae.fr/hal-05438697 ; Second Thematic Workshop on 3D Cell Models in Loire Valley, Nov 2025, Tours, France |
| Publisher Information: |
CCSD |
| Publication Year: |
2025 |
| Collection: |
Université François-Rabelais de Tours: HAL |
| Subject Terms: |
Salmonella Infections; Organoids; Intestinal Health; Chicken; [SDV]Life Sciences [q-bio] |
| Subject Geographic: |
Tours; France |
| Description: |
International audience ; Eggs and poultry meat are the major source of animal proteins worldwide. Salmonella Enteritidis (SE) is a major poultry products contaminant responsible for foodborne outbreaks (PMID: 39659847). It is thus important to find means of reduction of Salmonella colonization in poultry. One of the most promising approaches is the microbiota-based competitive exclusion to outcompete the pathogen in the chicken caecum, the intestinal site of SE persistence and shedding. However, the selection of microbiota bacterial components, such as the Mix4 oligo-consortium orally administered in chicks associated with reduction of Salmonella shedding (PMID: 40079593), requires an in vitro screening model to (i) reduce animal experiments, (ii) resemble in vivo environment, and (iii) allow to decipher mechanisms of protection. For that purpose, the utility of the chicken 3D and 2D caecum enteroids were evaluated. The first model was set up by deriving stem cells from day-old chick caecal crypts grown in Matrigel®, leading to 3D “apical in” enteroids (PMID: 37525204) whose growth and cellular organization was characterised under normoxia or physioxia. The second model was derived from the dissociation of 3D “apical in” enteroids and cell seeding under normoxia on a Transwell® membrane, leading to 2D “apical up” enteroids. The third model was set by deriving caecum villi from ED18 chick embryos according to a previously published procedure (PMID: 33742093), leading to “apical out” floating 3D enteroids that were infected with Salmonella Enteritidis and imaged under normoxia. This offers perspectives of deciphering mechanisms of host / commensal / pathogenic bacteria relationship to objectivate protective effect of a defined commensal consortium. |
| Document Type: |
conference object; still image |
| Language: |
English |
| Availability: |
https://hal.inrae.fr/hal-05438697; https://hal.inrae.fr/hal-05438697v1/document; https://hal.inrae.fr/hal-05438697v1/file/Poster_Augis_et_al-2ndWorkshopOrganoids_UnivTours-20251128.pdf |
| Rights: |
https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Accession Number: |
edsbas.64780502 |
| Database: |
BASE |