| Title: |
A Type IV-A CRISPR-Cas System in Pseudomonas aeruginosa Mediates RNA-Guided Plasmid Interference In Vivo |
| Authors: |
Crowley, Valerie M.; Catching, Adam; Taylor, Hannah N.; Borges, Adair L.; Metcalf, Josie; Bondy-Denomy, Joseph; Jackson, Ryan N. |
| Source: |
The CRISPR Journal ; volume 2, issue 6, page 434-440 ; ISSN 2573-1599 2573-1602 |
| Publisher Information: |
SAGE Publications |
| Publication Year: |
2019 |
| Description: |
Bacteria and archaea use CRISPR-Cas adaptive immune systems to destroy complementary nucleic acids using RNAs derived from CRISPR loci. Here, we provide the first functional evidence for type IV CRISPR-Cas, demonstrating that the system from Pseudomonas aeruginosa strain PA83 mediates RNA-guided interference against a plasmid in vivo , both clearing the plasmid and inhibiting its uptake. This interference depends on the putative NTP-dependent helicase activity of Csf4/DinG. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1089/crispr.2019.0048 |
| Availability: |
https://doi.org/10.1089/crispr.2019.0048; https://journals.sagepub.com/doi/full-xml/10.1089/crispr.2019.0048; https://journals.sagepub.com/doi/pdf/10.1089/crispr.2019.0048 |
| Rights: |
https://journals.sagepub.com/page/policies/text-and-data-mining-license |
| Accession Number: |
edsbas.647AF03B |
| Database: |
BASE |