| Title: |
O-077 The chromosomal constitution of embryos developing from 0PN zygotes |
| Authors: |
Gomez Peña, M; Papayannis, M; De Martino, E; Filardi, P; Magri, L; Chiarello, J M; Figueroa, C; De Zuñiga, I; Kopcow, L; Horton, M; Sobral, F; Oubiña, A; Bisioli, C |
| Source: |
Human Reproduction ; volume 37, issue Supplement_1 ; ISSN 0268-1161 1460-2350 |
| Publisher Information: |
Oxford University Press (OUP) |
| Publication Year: |
2022 |
| Description: |
Study question To compare the ploidy status of embryos originated from zygotes with absence of pronuclei (0PN) with those developing from normal two pronuclei (2PN) fertilization. Summary answer We did not find significant differences in euploidy rates between blastocysts from 0PN and 2PN zygotes. What is known already Information regarding the ploidy number of 0PN zygotes, even when the pronuclei number is assessed by time-lapse microscope coupled incubators, is still insufficient. Because of this, embryos originating from 0PN zygotes are usually discarded or individualized in order not to include them in first transfers. Even more, abnormal fertilization is considered as a proxy of poor embryo quality. Study design, size, duration A retrospective observational study from March 2016 and December 2021 including 80 PGT-A cycles in which at least one of the blastocysts studied developed from oocytes in which pronuclei were not seen at the time of fertilization check. Participants/materials, setting, methods 307 blastocysts were studied, of which 115 originated from 0PN and 192 from 2PN zygotes. Pronuclear number was assessed 16-20 h after insemination. Zygotes with 0PN were cultured separately from 2PN ones of the same cohort. After extended culture, a biopsy was performed and the material obtained was genetically studied using Next Generation Sequencing. Statistical comparisons were made with Fisher's exact and Chi-square tests. Significance level was set at P |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1093/humrep/deac104.091 |
| Availability: |
https://doi.org/10.1093/humrep/deac104.091; https://academic.oup.com/humrep/article-pdf/37/Supplement_1/deac104.091/44306457/deac104.091.pdf |
| Rights: |
https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model |
| Accession Number: |
edsbas.6678F007 |
| Database: |
BASE |