| Title: |
Prevalence of blood and skin trypanosomes in domestic and wild fauna from two sleeping sickness foci in Southern Cameroon |
| Authors: |
Magang, Eugenie Melaine Kemta; Kamga, Rolin Mitterran Ndefo; Telleria, Jenny; Tichit, Magali; Crouzols, Aline; Kaboré, Jacques; Hardy, David; Bouaka, Calmes Ursain Tsakeng; Jamonneau, Vincent; Rotureau, Brice; Kuete, Victor; Bart, Jean-Mathieu; Simo, Gustave |
| Contributors: |
Université de Dschang Cameroun = University of Dschang Cameroon (UDs); Interactions hôtes-vecteurs-parasites-environnement dans les maladies tropicales négligées dues aux trypanosomatides (UMR INTERTRYP); Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université Claude Bernard Lyon 1 (UCBL); Université de Lyon-Université de Lyon-Université de Bordeaux (UB); Plate-Forme d’Histopathologie / Histopathology Platform; Institut Pasteur Paris (IP)-Université Paris Cité (UPCité); Biologie cellulaire des Trypanosomes - Trypanosome Cell Biology; Institut Pasteur Paris (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité); Centre international de recherche-développement sur l'élevage en zone subhumide (CIRDES); Centre for Research in Infectious Diseases Yaoundé (CRID); Institut Pasteur de Guinée; Pasteur Network (Réseau International des Instituts Pasteur); This publication was made possible through the ARTS (Allocation de Recherche pour une Thèse au Sud) support provided by the IRD-DPF.; This work was supported in part by IRD internal grants (to JMB), IRD fellowship (ARTS to EMMK), the Institut Pasteur (to BR) and the French National Agency for Scientific Research (project ANR-18-CE15-0012 TrypaDerm to BR and JMB). It was also supported through the Human Heredity and Health in Africa (H3Africa; Grant ID H3A-18-004 to GS) from the science for Africa Foundation. H3Africa is jointly supported by Wellcome Trust and the National Institutes of Health (NIH). We acknowledge additional support from the the Royal Society of Tropical Medicine and Hygiene (RSTMH) small grant 2021 (No. 0021510 to EMMK) that funded through the National Institute for Health Research (NIHR) and the International Foundation for Science (I3-B-6687-1 to EMMK).The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.; We thank the breeders of Campo and Bipindi HAT foci who put at our disposal their animals for the realization of this study.; ANR-18-CE15-0012,TrypaDerm,Comprendre la biologie des trypanosomes africains dans la peau(2018) |
| Source: |
ISSN: 1935-2727. |
| Publisher Information: |
CCSD; Public Library of Science |
| Publication Year: |
2023 |
| Subject Terms: |
[SDV]Life Sciences [q-bio] |
| Description: |
International audience ; Although studies on African Trypanosomiases revealed a variety of trypanosome species in the blood of various animal taxa, animal reservoirs of Trypanosoma brucei gambiense and anatomical niches such as skin have been overlooked in most epidemiological settings. This study aims to update epidemiological data on trypanosome infections in animals from human African trypanosomiasis (HAT) foci of Cameroon. Blood and skin snips were collected from 291 domestic and wild animals. DNA was extracted from blood and skin snips and molecular approaches were used to identify different trypanosomes species. Immunohistochemical analyses were used to confirm trypanosome infections in skin snips. PCR revealed 137 animals (47.1%) with at least one trypanosome species in the blood and/or in the skin. Of these 137 animals, 90 (65.7%) and 32 (23.4%) had trypanosome infections respectively in the blood and skin. Fifteen (10.9%) animals had trypanosome infections in both blood and skin snip. Animals from the Campo HAT focus (55.0%) were significantly (X 2 = 17.6; P< 0.0001) more infected than those (29.7%) from Bipindi. Trypanosomes of the subgenus Trypanozoon were present in 27.8% of animals while T . vivax , T . congolense forest type and savannah type were detected in 16.5%, 10.3% and 1.4% of animals respectively. Trypanosoma b . gambiense infections were detected in the blood of 7.6% (22/291) of animals. No T . b . gambiense infection was detected in skin. This study highlights the presence of several trypanosome species in the blood and skin of various wild and domestic animals. Skin appeared as an anatomical reservoir for trypanosomes in animals. Despite methodological limitations, pigs, sheep, goats and wild animals were confirmed as potential reservoirs of T . b . gambiense . These animal reservoirs must be considered for the designing of control strategies that will lead to sustainable elimination of HAT. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/37498955; PUBMED: 37498955; PUBMEDCENTRAL: PMC10411957 |
| DOI: |
10.1371/journal.pntd.0011528 |
| Availability: |
https://pasteur.hal.science/pasteur-04182193; https://pasteur.hal.science/pasteur-04182193v1/document; https://pasteur.hal.science/pasteur-04182193v1/file/journal.pntd.0011528.pdf; https://doi.org/10.1371/journal.pntd.0011528 |
| Rights: |
http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.66C2B026 |
| Database: |
BASE |