| Title: |
Potential biomarkers for multiple sclerosis stage from targeted proteomics and microRNA sequencing |
| Authors: |
Tan, Ineke L; Modderman, Rutger; Stachurska, Anna; Almeida, Rodrigo; de Vries, Riemer; Heersema, Dorothea J; Gacesa, Ranko; Wijmenga, Cisca; Jonkers, Iris H; Meilof, Jan F; Withoff, Sebo |
| Source: |
Tan, I L, Modderman, R, Stachurska, A, Almeida, R, de Vries, R, Heersema, D J, Gacesa, R, Wijmenga, C, Jonkers, I H, Meilof, J F & Withoff, S 2024, 'Potential biomarkers for multiple sclerosis stage from targeted proteomics and microRNA sequencing', Brain Communications, vol. 6, no. 4, fcae209. https://doi.org/10.1093/braincomms/fcae209 |
| Publication Year: |
2024 |
| Collection: |
University of Groningen research database |
| Description: |
Multiple sclerosis is a chronic demyelinating disease of the central nervous system. There is a need for new circulating biomarkers for multiple sclerosis, in particular, markers that differentiate multiple sclerosis subtypes (relapsing-remitting, secondary progressive and primary progressive multiple sclerosis), as this can help in making treatment decisions. In this study, we explore two classes of potential multiple sclerosis biomarkers-proteins and microRNAs-circulating in the cerebrospinal fluid and serum. Targeted medium-throughput proteomics (92 proteins) and microRNA sequencing were performed on serum samples collected in a cross-sectional case-control cohort (cohort I, controls n = 30, multiple sclerosis n = 75) and a prospective multiple sclerosis cohort (cohort II, n = 93). For cohort I, we also made these measurements in paired cerebrospinal fluid samples. In the cohort I cerebrospinal fluid, we observed differences between multiple sclerosis and controls for 13 proteins, including some previously described to be markers for multiple sclerosis [e.g. CD27, C-X-C motif chemokine 13 (CXCL13) and interleukin-7 (IL7)]. No microRNAs were significantly differentially expressed between multiple sclerosis and controls in the cerebrospinal fluid. In serum, 10 proteins, including angiopoietin-1 receptor (TIE2), and 16 microRNAs were significantly different between relapsing-remitting multiple sclerosis and secondary progressive multiple sclerosis after performing a meta-analysis combining both cohorts. In the prospective part of the study, participants with relapsing-remitting multiple sclerosis were followed for around 3 years, during which time 12 participants converted to secondary progressive multiple sclerosis. In these longitudinally collected serum samples, we observed a peak in granzyme B, A and H proteins around the time of conversion. Single-sample enrichment analysis of serum microRNA profiles revealed that the peak in granzyme B levels around conversion coincides with enrichment for microRNAs that ... |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
English |
| ISSN: |
2632-1297 |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/38978729; info:eu-repo/semantics/altIdentifier/hdl/https://hdl.handle.net/11370/2eef0de8-9511-43ee-979c-c39a3a80dd5b; info:eu-repo/semantics/altIdentifier/pissn/2632-1297 |
| DOI: |
10.1093/braincomms/fcae209 |
| Availability: |
https://hdl.handle.net/11370/2eef0de8-9511-43ee-979c-c39a3a80dd5b; https://research.rug.nl/en/publications/2eef0de8-9511-43ee-979c-c39a3a80dd5b; https://doi.org/10.1093/braincomms/fcae209; https://pure.rug.nl/ws/files/1062328991/fcae209.pdf |
| Rights: |
info:eu-repo/semantics/openAccess ; http://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.6762368C |
| Database: |
BASE |