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Spatiotemporal dynamics and regulation of suture morphology and collagen remodeling during trans-sutural distraction osteogenesis

Title: Spatiotemporal dynamics and regulation of suture morphology and collagen remodeling during trans-sutural distraction osteogenesis
Authors: Zhiyu Lin; Yujie Chen; Peiyang Zhang; Mengying Jin; Yan Zha; Zhenmin Zhao
Source: npj Regenerative Medicine, Vol 11, Iss 1, Pp 1-16 (2026)
Publisher Information: Nature Portfolio
Publication Year: 2026
Collection: Directory of Open Access Journals: DOAJ Articles
Subject Terms: Medicine
Description: Trans-sutural distraction osteogenesis (TSDO) is an effective treatment of midfacial hypoplasia, a craniofacial deformity frequently associated with cleft lip and palate. Though extracellular matrix (ECM) remodeling plays a pivotal role in craniofacial correction, the characteristics and mechanisms underlying collagen reorganization and cellular morphological adaptations during TSDO remain poorly understood. This study quantitatively delineates the spatiotemporal changes of sutural cells and ECM morphology, revealing a polarized alignment parallel to the direction of mechanical force. Multi-omics analysis demonstrates that macrophages regulate collagen remodeling in suture mesenchymal stem cells (SuSCs) via the PDGF signaling pathway. Subsequent in vitro stretch loading models confirmed PDGF pathway activation enhances SuSCs migration, collagen synthesis, and cellular morphological reorganization. Validation in macrophage-elimination murine models further corroborated this regulatory axis. Collectively, our work maps the dynamic microenvironmental changes during TSDO and elucidates cell-cell interaction-driven ECM collagen remodeling. These insights advance the understanding of TSDO-mediated osteogenesis and provide a foundation for developing optimized therapeutic strategies.
Document Type: article in journal/newspaper
Language: English
Relation: https://doi.org/10.1038/s41536-025-00448-5; https://doaj.org/toc/2057-3995; https://doaj.org/article/259ebdc075e54293b862ac1842b45b52
DOI: 10.1038/s41536-025-00448-5
Availability: https://doi.org/10.1038/s41536-025-00448-5; https://doaj.org/article/259ebdc075e54293b862ac1842b45b52
Accession Number: edsbas.67962839
Database: BASE