| Title: |
Upregulation of miR-4286 and miR-146a-5p in Metastatic Melanoma, Revealed by Multiplex Expression Analysis |
| Authors: |
Iliyan Pochileev; Albena Fakirova; Desislava Tashkova; Aleksandra Gerdgikova; Nevena Ilieva; Denitsa Serteva; Gergana Shalamanova; Hristo Ivanov; Aleksandar Linev; Ivanka Dimova |
| Source: |
Current Issues in Molecular Biology ; Volume 48 ; Issue 3 ; Pages: 279 |
| Publisher Information: |
Multidisciplinary Digital Publishing Institute |
| Publication Year: |
2026 |
| Collection: |
MDPI Open Access Publishing |
| Subject Terms: |
miRNA; metastatic melanoma; BRAF; microRNA biomarkers; NanoString technology; miR-146a-5p; miR-4286 |
| Subject Geographic: |
agris |
| Description: |
Background: Metastatic melanoma is an extremely aggressive malignancy with limited therapeutic options, despite advances in targeted and immunotherapy. MicroRNAs are key post-transcriptional regulators of gene expression and play a critical role in tumor adaptation, invasion, and metastasis. The aim of our study was to identify dysregulated miRNAs which may serve as novel biomarkers and therapeutic targets. Materials and Methods: The study was conducted on FFPE samples from metastatic melanoma (n = 15), compared to healthy skin tissue (n = 6). BRAF V600E/Ec mutation status was established by Real-Time qPCR. Expression miRNA analysis was performed, using digital counting of 827 miRNAs on the NanoString platform, with data normalization and fold change calculations. Results: Following normalization and quality control metrics, 58 differentially expressed miRNAs were identified in BRAFwt melanoma samples: 6 overexpressed and 52 inderexpressed miRNAs. In BRAFmut melanoma, 37 microRNAs were differentially expressed: 11 overexpressed and 26 underexpressed. Four miRNAs showed elevated expression in both melanoma groups. Among them, miR-146a-5p and miR-4286 demonstrated the highest elevation, especially in BRAFmut tumors. We focused further on their targeted genes. Conclusion: This study demonstrates significant alterations in the miRNA expression profile in metastatic melanoma and highlights the potential of miR-146a-5p and miR-4286 as key regulators of tumor biology. |
| Document Type: |
text |
| File Description: |
application/pdf |
| Language: |
English |
| Relation: |
Bioinformatics and Systems Biology; https://dx.doi.org/10.3390/cimb48030279 |
| DOI: |
10.3390/cimb48030279 |
| Availability: |
https://doi.org/10.3390/cimb48030279 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.69409B60 |
| Database: |
BASE |