| Title: |
Food-borne microbes influence conjugative transfer of antimicrobial resistance plasmids in pre-disturbed gut microbiome |
| Authors: |
Ottenbrite, Marie; Yilmaz, Gokhan; Chan, Maria; Devenish, John; Kang, Mingsong; Dan, Hanhong; Lau, Calvin Ho-Fung; Capitani, Sabrina; Carrillo, Catherine; Bessonov, Kyrylo; Nash, John H.E.; Topp, Edward; Guan, Jiewen |
| Contributors: |
Government of Canada Shared Priority Project, Genomics Research and Development Initiative |
| Source: |
Canadian Journal of Microbiology ; volume 71, page 1-11 ; ISSN 0008-4166 1480-3275 |
| Publisher Information: |
Canadian Science Publishing |
| Publication Year: |
2025 |
| Description: |
Ingestion of antibiotic-resistant bacteria following antibiotic treatments may lead to the transfer of antimicrobial resistance genes (ARGs) within a disturbed gut microbiota. However, it remains unclear whether and how microbes present in food matrices influence ARG transfer. Thus, a previously established mouse model, which demonstrated the conjugative transfer of a multi-drug resistance plasmid (pIncA/C) from Salmonella Heidelberg (donor) to Salmonella Typhimurium (recipient), was used to assess the effects of food-borne microbes derived from fresh carrots on pIncA/C transfer. Mice were pre-treated with ampicillin, streptomycin, sulfamethazine, or left untreated as a control to facilitate bacterial colonization. Contrary to previous findings where high-density colonization of the donor and recipient bacteria occurred in the absence of food-borne microbes, the presence of these microbes resulted in a low abundance of S. Typhimurium and no detection of S. Typhimurium transconjugants in the fecal samples from any of the mice. However, in mice pre-treated with streptomycin, a significant reduction in microbial species richness allowed for the significant enrichment of Enterobacteriaceae and pIncA/C transfer to bacteria from the genera Escherichia, Enterobacter, Citrobacter, and Proteus. These findings suggest that food-borne microbes may enhance ARG dissemination by influencing the population dynamics of bacterial hosts within a pre-disturbed gut microbiome. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1139/cjm-2024-0168 |
| Availability: |
https://doi.org/10.1139/cjm-2024-0168; https://cdnsciencepub.com/doi/full-xml/10.1139/cjm-2024-0168; https://cdnsciencepub.com/doi/pdf/10.1139/cjm-2024-0168 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/deed.en_GB |
| Accession Number: |
edsbas.6999C42F |
| Database: |
BASE |