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Evaluation of sugar content and bioethanol production of Ethiopian local varieties “Nech Tinkish” and “Hawaye” sweet sorghum (Sorghum bicolor (L.))

Title: Evaluation of sugar content and bioethanol production of Ethiopian local varieties “Nech Tinkish” and “Hawaye” sweet sorghum (Sorghum bicolor (L.))
Authors: Kasegn, Melaku Mekonen; Simachew, Addis; Redda, Yisehak Tsegaye; Gebremedhn, Hailay Mehari; Berhanu, Addisu Desalegn; Mossa, Mohammed Mebrahtu
Source: Biotechnology for the Environment ; volume 1, issue 1 ; ISSN 2948-2356
Publisher Information: Springer Science and Business Media LLC
Publication Year: 2024
Description: Diversifying the use of climate-smart crops such as sweet sorghum has the potential to solve integrated food, bioenergy, feed, and land management problems . The study’s purpose is to quantify the sugar content of Nech Tinkish (v1) and Hawaye (v2) Ethiopian sweet sorghum varieties and investigate the interaction effect of fermentation parameters to determine their capacity for ethanol production. Sweet sorghum varieties were analyzed to determine their difference in °Brix content by extracting their juices. The juice was clarified using milk lime. Its total soluble sugars, total carbohydrates, and reducing sugars were determined using a digital refractometer, phenol sulfuric acid, and 3,5-dinitrosalicylic acid, respectively. A completely randomized factorial was employed to evaluate ethanol production capacity, and the ethanol content was estimated using a potassium dichromate solution. The °Brix results revealed that v2 had a higher sugar concentration than v1. Additionally, the estimated carbohydrate content of the juice ranged from 37.402 to 157.641 g/L. The estimated reducing sugar also varied from 4.644 to 33.412 g/L. Therefore, the estimated reducing sugar showed the hydrolysis of sweet sorghum juice by invertase and sulfuric acid produced more fermentable sugars. Fermentation at 30 °C with pH 4.5 incubated for 4 days yields the highest ethanol, and v2 yields higher (15.31%) ethanol, compared to v1 produced 14.85%. This study showed a basis for the existence of two sugar-rich climate smart sweet sorghum varieties with an extraordinary amount of sugar used as a source of biofuel and food simultaneously in a single plot of land.
Document Type: article in journal/newspaper
Language: English
DOI: 10.1186/s44314-024-00001-6
DOI: 10.1186/s44314-024-00001-6.pdf
DOI: 10.1186/s44314-024-00001-6/fulltext.html
Availability: http://dx.doi.org/10.1186/s44314-024-00001-6; https://link.springer.com/content/pdf/10.1186/s44314-024-00001-6.pdf; https://link.springer.com/article/10.1186/s44314-024-00001-6/fulltext.html
Rights: https://creativecommons.org/licenses/by/4.0 ; https://creativecommons.org/licenses/by/4.0
Accession Number: edsbas.6B2F702A
Database: BASE