| Title: |
Truncated PNA-Functionalized Porous Silicon Biosensor for Low-Cost and Early Detection of Troponin T in Myocardial Infarction |
| Authors: |
Maria Grazia Nolli; Alessio Bartocci; Caterina De Rosa; Andrea Patrizia Falanga; Vincenzo Abbate; Monica Terracciano; Valeria Nocerino; Ilaria Rea; Luca De Stefano; Sabrina Giordano; Gennaro Piccialli; Marco Fabio Costantino; Carminia Maria Della Corte; Elise Dumont; Nicola Borbone; Giovanni Orabona Dell’Aversana; Giorgia Oliviero |
| Contributors: |
Nolli, Maria Grazia; Bartocci, Alessio; De Rosa, Caterina; Falanga, Andrea Patrizia; Abbate, Vincenzo; Terracciano, Monica; Nocerino, Valeria; Rea, Ilaria; De Stefano, Luca; Giordano, Sabrina; Piccialli, Gennaro; Fabio Costantino, Marco; Maria Della Corte, Carminia; Dumont, Elise; Borbone, Nicola; Dell'Aversana Orabona, Giovanni; Oliviero, Giorgia |
| Publication Year: |
2026 |
| Collection: |
IRIS Università degli Studi di Napoli Federico II |
| Subject Terms: |
in silico study; PNA bio-probe; PSi optical transducer; surface chemical functionalization; label-free cardiac troponin T detection |
| Description: |
A label-free optical biosensor based on porous silicon (PSi) was developed for the high-sensitivity detection of human cardiac troponin T (cTnT), a key biomarker for the early diagnosis of myocardial infarction (MI). To reduce synthesis costs while maintaining binding efficiency, a truncated peptide nucleic acid (PNA) probe was designed in silico starting from a 40-base-long wild-type sequence. Computational screening identified a 12-base candidate, whose binding affinity was experimentally validated by western blot analysis. The freshly etched PSi surface was passivated and functionalized via mild thermal hydrosilylation with 10-undecenoic acid to introduce carboxylic groups, enabling covalent PNA immobilization through the carbodiimide chemistry. Surface functionalization and stability in aqueous media were validated using both label-free optical reflectance and fluorescence spectroscopy. Probe conjugation was optimized at pH 5.5, resulting in a surface density of 1.12 ± 0.30 pmol cm−2. The biosensor exhibited reproducible, concentration-dependent responses to cTnT in the 0.02–0.16 ng mL–1 range, with a limit of detection of 30 ± 0.02 pg mL–1. This performance is clinically relevant, as cTnT levels in healthy individuals are typically 100 ng mL–1 in severe cases. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
volume:299; firstpage:129054; numberofpages:13; journal:TALANTA; https://hdl.handle.net/11588/1016090; https://www.sciencedirect.com/science/article/pii/S0039914025015450?via=ihub |
| DOI: |
10.1016/j.talanta.2025.129054 |
| Availability: |
https://hdl.handle.net/11588/1016090; https://doi.org/10.1016/j.talanta.2025.129054; https://www.sciencedirect.com/science/article/pii/S0039914025015450?via=ihub |
| Rights: |
info:eu-repo/semantics/openAccess ; license:Creative commons ; license uri:http://creativecommons.org/licenses/by-sa/4.0/ |
| Accession Number: |
edsbas.6B62029A |
| Database: |
BASE |