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Faecal microbiome, gastrointestinal integrity, inflammation and thermoregulation in recent exertional heat illness patients and matched controls

Title: Faecal microbiome, gastrointestinal integrity, inflammation and thermoregulation in recent exertional heat illness patients and matched controls
Authors: Gould, AAM; Walsh, NP; Tipton, MJ; Zurawlew, MJ; Tayari, O; House, C; Delves, SK; Robson, SC; Shute, JJ; Watts, JEM; Roberts, AJ; Rawcliffe, AJ; Robinson, MR; Corbett, J
Publisher Information: Wiley
Publication Year: 2025
Collection: Liverpool John Moores University: LJMU Research Online
Subject Terms: RC1200 Sports Medicine
Description: The gastrointestinal (GI) microbiota and GI barrier integrity are hypothesised to contribute to exertional heat illness (EHI) aetiology. We compared the faecal microbiome, GI barrier integrity, inflammation and thermoregulation of 29 recent (∼4 months) EHI patients (a group with elevated EHI risk) and 29 control individuals without prior EHI history, matched for variables influencing thermoregulation and GI microbiota. Participants completed an exercise heat tolerance assessment (HTA), with faecal microbiome assessed by 16S rRNA gene amplicon sequencing of stool samples and blood biomarkers of GI barrier integrity and inflammation measured pre‐ and post‐HTA. With the exception of the Simpson index (patient = 0.97 ± 0.01 vs. control = 0.98 ± 0.00, P = 0.030), there were no between‐groups differences in faecal microbiome composition (α‐diversity, β‐diversity, relative abundance, differential abundance), GI barrier integrity, inflammation or terminal thermoregulatory indices. Individuals were subsequently classified as heat tolerant (n = 46) or intolerant (n = 12) on the basis of the HTA. Heat intolerant individuals demonstrated lower sudomotor response (intolerant = 0.53 (0.17) vs. tolerant = 0.62 (0.20) L m−2 h−1, P = 0.011) despite greater thermoregulatory strain (e.g., terminal Trec: intolerant = 39.20 ± 0.31 vs. tolerant = 38.80 ± 0.31°C, P < 0.001), lower Firmicutes:Bacteroidota ratio (intolerant = 3.7 (0.6) vs. tolerant = 4.5 (2.0), P = 0.019) and higher plasma [sCD14] (P = 0.014), but other aspects of faecal microbiome, GI integrity or inflammation did not differ from heat tolerant individuals. In ...
Document Type: article in journal/newspaper
File Description: text
Language: English
ISSN: 0958-0670
Relation: https://researchonline.ljmu.ac.uk/id/eprint/26674/1/Faecal%20microbiome%20gastrointestinal%20integrity%20inflammation%20and%20thermoregulation.pdf; Gould, AAM, Walsh, NP, Tipton, MJ, Zurawlew, MJ ORCID logoorcid:0000-0003-3608-1028 , Tayari, O, House, C, Delves, SK, Robson, SC, Shute, JJ, Watts, JEM, Roberts, AJ, Rawcliffe, AJ, Robinson, MR and Corbett, J ORCID logoorcid:0000-0002-6552-6471 (2025) Faecal microbiome, gastrointestinal integrity, inflammation and thermoregulation in recent exertional heat illness patients and matched controls. Experimental Physiology. ISSN 0958-0670
DOI: 10.1113/ep092849
Availability: https://researchonline.ljmu.ac.uk/id/eprint/26674/; https://researchonline.ljmu.ac.uk/id/eprint/26674/1/Faecal%20microbiome%20gastrointestinal%20integrity%20inflammation%20and%20thermoregulation.pdf; https://doi.org/10.1113/ep092849
Rights: cc_by
Accession Number: edsbas.6D85A2CF
Database: BASE