| Title: |
Comparative Genome-Centric Analysis of Freshwater and Marine ANAMMOX Cultures Suggests Functional Redundancy in Nitrogen Removal Processes |
| Authors: |
Ali, Muhammad; Shaw, Dario Rangel; Albertsen, Mads; Saikaly, Pascal |
| Contributors: |
Biological and Environmental Sciences and Engineering (BESE) Division; Environmental Biotechnology Research Group; Environmental Science and Engineering Program; Water Desalination and Reuse Center (WDRC), Biological and Environmental Science Engineering (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia; Water Desalination and Reuse Research Center (WDRC); Center for Microbial Communities, Aalborg University, Aalborg, Denmark |
| Publisher Information: |
Frontiers Media SA |
| Publication Year: |
2020 |
| Collection: |
King Abdullah University of Science and Technology: KAUST Repository |
| Description: |
There is a lack of understanding of the interaction between anammox bacteria and the flanking microbial communities in both freshwater (non-saline) and marine (saline) ecosystems. Here, we present a comparative genome-based exploration of two different anammox bioreactors, through the analysis of 23 metagenome-assembled genomes (MAGs), 12 from freshwater anammox reactor (FWR), and 11 from marine anammox reactor (MWR). To understand the contribution of individual members to community functions, we applied the index of replication (iRep) to determine bacteria that are actively replicating. Using genomic content and iRep information, we provided a potential ecological role for the dominant members of the community based on the reactor operating conditions. In the non-saline system, anammox (Candidatus Brocadia sinica) and auxotrophic neighboring bacteria belonging to the phyla Ignavibacteriae and Chloroflexi might interact to reduce nitrate to nitrite for direct use by anammox bacteria. Whereas, in the saline reactor, anammox bacterium (Ca. Scalindua erythraensis) and flanking community belonging to phyla Planctomycetes (different than anammox bacteria)—which persistently growing in the system—may catabolize detritus and extracellular material and recycle nitrate to nitrite for direct use by anammox bacteria. Despite different microbial communities, there was functional redundancy in both ecosystems. These results signify the potential application of marine anammox bacteria for treating saline N-rich wastewaters. ; Funding. This work was supported by Center Competitive Funding Program (FCC/1/ 1971-05-01) from King Abdullah University of Science and Technology (KAUST). MAlb was supported by a research grant (15510) from VILLUM FONDEN. |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
unknown |
| ISSN: |
1664-302X |
| Relation: |
https://www.frontiersin.org/article/10.3389/fmicb.2020.01637/full; https://www.frontiersin.org/articles/10.3389/fmicb.2020.01637/pdf; Ali, M., Shaw, D. R., Albertsen, M., & Saikaly, P. E. (2020). Comparative Genome-Centric Analysis of Freshwater and Marine ANAMMOX Cultures Suggests Functional Redundancy in Nitrogen Removal Processes. Frontiers in Microbiology, 11. doi:10.3389/fmicb.2020.01637; 2-s2.0-85088465535; Frontiers in Microbiology; http://hdl.handle.net/10754/664524; 11 |
| DOI: |
10.3389/fmicb.2020.01637 |
| Availability: |
http://hdl.handle.net/10754/664524; https://doi.org/10.3389/fmicb.2020.01637 |
| Rights: |
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. ; https://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.6DA63F04 |
| Database: |
BASE |