| Title: |
Dominant role of Ste12 DNA binding domain for PRE conformation selection. |
| Authors: |
Sandrine Pinheiro; Mariona Nadal-Ribelles; Carme Solé; Vincent Vincenzetti; Yves Dusserre; Francesc Posas; Serge Pelet |
| Publication Year: |
2025 |
| Collection: |
The University of Auckland: Figshare |
| Subject Terms: |
Microbiology; Cell Biology; Genetics; Molecular Biology; Immunology; Developmental Biology; Science Policy; Infectious Diseases; Plant Biology; Biological Sciences not elsewhere classified; two haploid cells; saccharomyces cerevisiae < p; core promoter modulate; ste12 binding sites; binding sites; strong dimer; specific timing; largely encoded; large diversity; basal association |
| Description: |
A. Schematic of the Ste12 transcription factor composed of a DNA-binding domain and a flexible activation domain. The small schemes describe the diversity of Ste12 homodimer interactions that could take place when varying the orientation or the distances of the PRE sites on the promoter. B. Scheme of the Ste12-EV chimeric transcription factor where the activation domain of Ste12 has been replaced by the estrogen receptor and the VP16 activation domain resulting in an β-estradiol responsive TF. C. , D. Dynamics of nuclear relocation following stimulation with 1µM β-estradiol at time 0 for strains containing the p AGA1 -dPSTR Y (C) and various p SYN -dPSTR R (D) . The solid lines represent the median of the population and the shaded area, the 25- to 75- percentiles. E. Comparison of the inducibility of various PRE conformation with the Ste12 WT or the Ste12-EV (black borders). The Expression Outputs (EO) for the synthetic reporters induced by the Ste12 WT or the Ste12-EV were normalized relative to the EO of the reference p SYN 3TT . The bar represents the mean response of the replicates shown by the circles. A significant difference between the normalized EO of Ste12-WT and Ste12-EV based on the mean of the individual replicates is indicated by a star (t-test: p-val < 0.05). |
| Document Type: |
still image |
| Language: |
unknown |
| Relation: |
https://figshare.com/articles/figure/Dominant_role_of_Ste12_DNA_binding_domain_for_PRE_conformation_selection_/29332276 |
| DOI: |
10.1371/journal.pgen.1011710.g002 |
| Availability: |
https://doi.org/10.1371/journal.pgen.1011710.g002; https://figshare.com/articles/figure/Dominant_role_of_Ste12_DNA_binding_domain_for_PRE_conformation_selection_/29332276 |
| Rights: |
CC BY 4.0 |
| Accession Number: |
edsbas.6E881580 |
| Database: |
BASE |