| Title: |
BAI1 localizes AMPA receptors at the cochlear afferent post-synaptic density and is essential for hearing |
| Authors: |
Carlton, Adam J; Jeng, Jing-Yi; Grandi, Fiorella C; De Faveri, Francesca; Amariutei, Ana E; De Tomasi, Lara; O'Connor, Andrew; Johnson, Stuart L; Furness, David N; Brown, Steve DM; Ceriani, Federico; Bowl, Michael R; Mustapha, Mirna; Marcotti, Walter |
| Source: |
Cell Reports , 43 (4) , Article 114025. (2024) |
| Publisher Information: |
CELL PRESS |
| Publication Year: |
2024 |
| Collection: |
University College London: UCL Discovery |
| Subject Terms: |
hair cell; development; ribbon synapses; afferent fibers; glutamate receptors; hearing loss; NDMA receptors; cochlea; post-synaptic density |
| Description: |
Type I spiral ganglion neurons (SGNs) convey sound information to the central auditory pathway by forming synapses with inner hair cells (IHCs) in the mammalian cochlea. The molecular mechanisms regulating the formation of the post-synaptic density (PSD) in the SGN afferent terminals are still unclear. Here, we demonstrate that brain-specific angiogenesis inhibitor 1 (BAI1) is required for the clustering of AMPA receptors GluR2–4 (glutamate receptors 2–4) at the PSD. Adult Bai1-deficient mice have functional IHCs but fail to transmit information to the SGNs, leading to highly raised hearing thresholds. Despite the almost complete absence of AMPA receptor subunits, the SGN fibers innervating the IHCs do not degenerate. Furthermore, we show that AMPA receptors are still expressed in the cochlea of Bai1-deficient mice, highlighting a role for BAI1 in trafficking or anchoring GluR2–4 to the PSDs. These findings identify molecular and functional mechanisms required for sound encoding at cochlear ribbon synapses. |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
English |
| Relation: |
https://discovery.ucl.ac.uk/id/eprint/10206893/ |
| Availability: |
https://discovery.ucl.ac.uk/id/eprint/10206893/1/BAI1%20localizes%20AMPA%20receptors%20at%20the%20cochlear%20afferent%20post-synaptic%20density%20and%20is%20essential%20for%20hearing.pdf; https://discovery.ucl.ac.uk/id/eprint/10206893/ |
| Rights: |
open |
| Accession Number: |
edsbas.70A6F237 |
| Database: |
BASE |