| Title: |
Generation and Characterization of Typhoid Toxin-Neutralizing Human Monoclonal Antibodies |
| Authors: |
Jiao, Xuyao; Smith, Sarah; Stack, Gabrielle; Liang, Qi; Bradley, Allan; Kellam, Paul; Galán, Jorge E. |
| Contributors: |
Torres, Victor J.; HHS | NIH | National Institute of Allergy and Infectious Diseases; Bill and Melinda Gates Foundation |
| Source: |
Infection and Immunity ; volume 88, issue 10 ; ISSN 0019-9567 1098-5522 |
| Publisher Information: |
American Society for Microbiology |
| Publication Year: |
2020 |
| Description: |
Typhoid toxin is a virulence factor of Salmonella enterica serovar Typhi, the causative agent of typhoid fever, and is thought to be responsible for the symptoms of severe disease. This toxin has a unique A2B5 architecture with two active subunits, the ADP ribosyl transferase PltA and the DNase CdtB, linked to a pentameric B subunit, which is alternatively made of PltB or PltC. Here, we describe the generation and characterization of typhoid toxin-neutralizing human monoclonal antibodies by immunizing genetically engineered mice that have a full set of human immunoglobulin variable region genes. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1128/iai.00292-20 |
| DOI: |
10.1128/IAI.00292-20 |
| Availability: |
https://doi.org/10.1128/iai.00292-20; https://journals.asm.org/doi/pdf/10.1128/IAI.00292-20 |
| Rights: |
https://journals.asm.org/non-commercial-tdm-license |
| Accession Number: |
edsbas.70A824 |
| Database: |
BASE |