| Title: |
Human herpesvirus-8 (HHV-8) sero-detection and HIV association in Kaposi's sarcoma (KS), non-KS tumors and non-neoplastic conditions |
| Authors: |
Pak Fatemeh; Kokhaei Parviz; Pyakurel Pawan; Mwakigonja Amos R; Lema Leonard K; Kaaya Ephata E; Biberfeld Peter |
| Source: |
Infectious Agents and Cancer, Vol 3, Iss 1, p 10 (2008) |
| Publisher Information: |
BMC |
| Publication Year: |
2008 |
| Collection: |
Directory of Open Access Journals: DOAJ Articles |
| Subject Terms: |
Neoplasms. Tumors. Oncology. Including cancer and carcinogens; RC254-282; Infectious and parasitic diseases; RC109-216 |
| Description: |
Background The association of the human herpesvirus-8/Kaposi's sarcoma (KS)-associated herpesvirus (HHV-8/KSHV) serology with various malignancies in Tanzania is not currently well established while previous studies were based on either PCR or immunofluorescence assays [IFA] but not with a sensitive enzyme-linked immunosorbent assay (ELISA). Selected archival diagnostic biopsies (n = 184) and sera from indigenous patients with KS (n = 120), non-KS tumors (n = 24) and non-neoplastic lesions (n = 40) at Muhimbili National Hospital (MNH), Tanzania, were evaluated by diagnostic histopathology, immunohistology [anti-HHV-8 latency-associated nuclear antigen (LANA)] and serology for HIV (ELISA) and HHV-8 (IFA and ELISA). Results About 66.3% (n = 122) cases including AIDS-associated Kaposi's sarcoma (AKS) (n = 93), reactive conditions (n = 28) and only one non-KS tumour were HIV positive. Endemic KS (EKS) patients were mostly males (96.3%, 26/27) who were less (69.9%, 65/93) predominant in AIDS-associated (AKS). A high (89%) percentage of patients with anti-HHV-8 antibodies was found in the cohort including the HIV positive (92%) cases, males (81.2%), KS patients (93%), non-KS tumors (92%), and reactive conditions (75%). All HHV-8 seronegative KS cases were nodular stage whereas both sera and corresponding biopsies from early stage KS were HHV-8+. Assay sensitivity, positive predictive value (PPV) and specificity were 98.6%, 93.5% and 16.7% for IFA and 93.5%, 98.6% and 50.0% for ELISA respectively. Conclusion HHV-8 seroprevalence at MNH appears high as expected among AKS cases and males but also in non-KS patients. ELISA showed a combination of high HHV-8 sensitivity as well as higher PPV and specificity than IFA which however, showed higher sensitivity. The apparent stage-dependent, inverted serum HHV-8 immunoreactivity supports a notion of viral immune-segregation during KS development. Routine HHV-8 screening should be considered particularly in patients at risk of KS and for selection of blood/organ ... |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
http://www.infectagentscancer.com/content/3/1/10; https://doaj.org/toc/1750-9378; https://doaj.org/article/c23e42fcfb884a9db3116303f4d3ab87 |
| DOI: |
10.1186/1750-9378-3-10 |
| Availability: |
https://doi.org/10.1186/1750-9378-3-10; https://doaj.org/article/c23e42fcfb884a9db3116303f4d3ab87 |
| Accession Number: |
edsbas.70AC5202 |
| Database: |
BASE |