| Title: |
An accessible, efficient and global approach for the large-scale sequencing of bacterial genomes |
| Authors: |
Perez-Sepulveda, BM; Heavens, D; Pulford, CV; Predeus, AV; Low, R; Webster, H; Dykes, GF; Schudoma, C; Rowe, W; Lipscombe, J; Watkins, C; Kumwenda, B; Shearer, N; Costigan, K; Baker, KS; Feasey, NA; Hinton, JCD; Hall, N |
| Publisher Information: |
BioMed Central |
| Publication Year: |
2026 |
| Collection: |
Oxford University Research Archive (ORA) |
| Description: |
We have developed an efficient and inexpensive pipeline for streamlining large-scale collection and genome sequencing of bacterial isolates. Evaluation of this method involved a worldwide research collaboration focused on the model organism Salmonella enterica, the 10KSG consortium. Following the optimization of a logistics pipeline that involved shipping isolates as thermolysates in ambient conditions, the project assembled a diverse collection of 10,419 isolates from low- and middle-income countries. The genomes were sequenced using the LITE pipeline for library construction, with a total reagent cost of less than USD$10 per genome. Our method can be applied to other large bacterial collections to underpin global collaborations. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1186/s13059-021-02536-3 |
| Availability: |
https://doi.org/10.1186/s13059-021-02536-3; https://ora.ox.ac.uk/objects/uuid:27aa05e2-eeac-4b27-a923-c6b7f87a52d0 |
| Rights: |
info:eu-repo/semantics/openAccess ; CC Attribution (CC BY) |
| Accession Number: |
edsbas.7140911B |
| Database: |
BASE |