| Title: |
Serological response in RT-PCR confirmed h1n1-2009 influenza a by hemagglutination inhibition and virus neutralization assays: An observational study |
| Authors: |
Chen, MI; Barr, IG; Koh, GCH; Lee, VJ; Lee, CPS; Shaw, R; Lin, C; Yap, J; Cook, AR; Tan, BH; Loh, JP; Barkham, T; Chow, VTK; Lin, RTP; Leo, YS |
| Contributors: |
Brown, J |
| Publisher Information: |
PUBLIC LIBRARY SCIENCE |
| Publication Year: |
2010 |
| Collection: |
The University of Melbourne: Digital Repository |
| Description: |
BACKGROUND: We describe the serological response following H1N1-2009 influenza A infections confirmed by reverse-transcriptase polymerase chain reaction (RT-PCR). METHODOLOGY AND PRINCIPAL FINDINGS: The study included patients admitted to hospital, subjects of a seroepidemiologic cohort study, and participants identified from outbreak studies in Singapore. Baseline (first available blood sample) and follow-up blood samples were analyzed for antibody titers to H1N1-2009 and recently circulating seasonal influenza A virus strains by hemagglutination inhibition (HI) and virus micro-neutralization (VM) assays. 267 samples from 118 cases of H1N1-2009 were analyzed. Geometric mean titers by HI peaked at 123 (95% confidence interval, CI 43-356) between days 30 to 39. The chance of observing seroconversion (four-fold or greater increase of antibodies) was maximized when restricting analysis to 45 participants with baseline sera collected within 5 days of onset and follow-up sera collected 15 or more days after onset; for these participants, 82% and 89% seroconverted to A/California/7/2009 H1N1 by HI and VM respectively. A four-fold or greater increase in cross-reactive antibody titers to seasonal A/Brisbane/59/2007 H1N1, A/Brisbane/10/2007 H3N2 and A/Wisconsin/15/2009 H3N2 occurred in 20%, 18% and 16% of participants respectively. CONCLUSIONS AND SIGNIFICANCE: Appropriately timed paired serology detects 80-90% RT-PCR confirmed H1N1-2009; Antibodies from infection with H1N1-2009 cross-reacted with seasonal influenza viruses. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| ISSN: |
1932-6203 |
| Relation: |
https://hdl.handle.net/11343/254631 |
| Availability: |
https://hdl.handle.net/11343/254631 |
| Rights: |
https://creativecommons.org/licenses/by/4.0 ; CC BY |
| Accession Number: |
edsbas.71450089 |
| Database: |
BASE |