| Title: |
Molecular alliance of Lymantria dispar multiple nucleopolyhedrovirus and a short unmodified antisense oligonucleotide of its anti-apoptotic IAP-3 gene: A novel approach for gypsy moth control |
| Authors: |
Oberemok, Volodymyr V.; Laikova, Kateryna V.; Zaitsev, Aleksei S.; Shumskykh, Maksym N.; Kasich, Igor N.; Gal’chinsky, Nikita V.; Bekirova, Viktoriya V.; Makarov, Valentin V.; Agranovsky, Alexey A.; Gushchin, Vladimir A.; Zubarev, Ilya V.; Kubyshkin, Anatoly V.; Fomochkina, Iryna I.; Gorlov, Mikhail V.; Skorokhod, Oleksii A. |
| Contributors: |
Oberemok, Volodymyr V.; Laikova, Kateryna V.; Zaitsev, Aleksei S.; Shumskykh, Maksym N.; Kasich, Igor N.; Gal’chinsky, Nikita V.; Bekirova, Viktoriya V.; Makarov, Valentin V.; Agranovsky, Alexey A.; Gushchin, Vladimir A.; Zubarev, Ilya V.; Kubyshkin, Anatoly V.; Fomochkina, Iryna I.; Gorlov, Mikhail V.; Skorokhod, Oleksii A. |
| Publication Year: |
2017 |
| Collection: |
Università degli studi di Torino: AperTo (Archivio Istituzionale ad Accesso Aperto) |
| Subject Terms: |
Baculovirus infection; DNA insecticide; Gypsy moth Lymantria dispar; Insect pest management; Multiple nucleopolyhedrovirus IAP (inhibitor-of-apoptosis) gene; RING (really interesting new gene); Animal; Apoptosi; Genes; Viral; Insect Control; Larva; Moth; Nucleopolyhedroviru; Transcriptome; Viral Protein; Oligodeoxyribonucleotides; Antisense; Catalysi; Molecular Biology; Spectroscopy; Computer Science Applications1707 Computer Vision and Pattern Recognition; Physical and Theoretical Chemistry; Organic Chemistry; Inorganic Chemistry |
| Description: |
Baculovirus IAP (inhibitor-of-apoptosis) genes originated by capture of host genes. Unmodified short antisense DNA oligonucleotides (oligoDNAs) from baculovirus IAP genes can down-regulate specific gene expression profiles in both baculovirus-free and baculovirus-infected insects. In this study, gypsy moth (Lymantria dispar) larvae infected with multiple nucleopolyhedrovirus (LdMNPV), and LdMNPV-free larvae, were treated with oligoDNA antisense to the RING (really interesting new gene) domain of the LdMNPV IAP-3 gene. The results with respect to insect mortality, biomass accumulation, histological studies, RT-PCR, and analysis of DNA apoptotic fragmentation suggest that oligoRING induced increased apoptotic processes in both LdMNPV-free and LdMNPV-infected insect cells, but were more pronounced in the latter. These data open up possibilities for promising new routes of insect pest control using antisense phosphodiester DNA oligonucleotides. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/29149051; info:eu-repo/semantics/altIdentifier/wos/WOS:000416811300217; volume:18; issue:11; firstpage:2446; lastpage:2462; numberofpages:16; journal:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; http://hdl.handle.net/2318/1688722; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85035043293; http://www.mdpi.com/1422-0067/18/11/2446/pdf |
| DOI: |
10.3390/ijms18112446 |
| Availability: |
http://hdl.handle.net/2318/1688722; https://doi.org/10.3390/ijms18112446; http://www.mdpi.com/1422-0067/18/11/2446/pdf |
| Rights: |
info:eu-repo/semantics/openAccess |
| Accession Number: |
edsbas.724CE98A |
| Database: |
BASE |