| Title: |
Optimization of fluorophores for chemical tagging and immunohistochemistry of Drosophila neurons. |
| Authors: |
Meissner, Geoffrey W; Grimm, Jonathan B; Johnston, Rebecca M; Sutcliffe, Ben; Ng, Julian; Jefferis, Gregory SXE; Cachero, Sebastian; Lavis, Luke D; Malkesman, Oz |
| Publisher Information: |
PLOS; //doi.org/10.1371/journal.pone.0200759 |
| Publication Year: |
2018 |
| Collection: |
Apollo - University of Cambridge Repository |
| Subject Terms: |
Animals; Genetically Modified; Brain; Drosophila melanogaster; Female; Fluorescent Dyes; Immunohistochemistry; Microscopy; Confocal; Molecular Structure; Neurons; Staining and Labeling |
| Description: |
The use of genetically encoded 'self-labeling tags' with chemical fluorophore ligands enables rapid labeling of specific cells in neural tissue. To improve the chemical tagging of neurons, we synthesized and evaluated new fluorophore ligands based on Cy, Janelia Fluor, Alexa Fluor, and ATTO dyes and tested these with recently improved Drosophila melanogaster transgenes. We found that tissue clearing and mounting in DPX substantially improves signal quality when combined with specific non-cyanine fluorophores. We compared and combined this labeling technique with standard immunohistochemistry in the Drosophila brain. ; This work was supported by Howard Hughes Medical Institute (https://www.hhmi.org), the Medical Research Council (https://mrc.ukri.org; MRC file reference U105188491) and a European Research Council (https://erc.europa.eu) Consolidator grant (649111) to G.S.X.E.J., and a Royal Society (https://royalsociety.org) Dorothy Hodgkin Fellowship to S.C. |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
English |
| Relation: |
https://www.repository.cam.ac.uk/handle/1810/290919 |
| DOI: |
10.17863/CAM.38098 |
| Availability: |
https://www.repository.cam.ac.uk/handle/1810/290919; https://doi.org/10.17863/CAM.38098 |
| Rights: |
Attribution 4.0 International ; https://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.7263BAD7 |
| Database: |
BASE |