| Title: |
β-1,6-Glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wall |
| Authors: |
Bekirian, Clara; Valsecchi, Isabel; Bachellier-Bassi, Sophie; Scandola, Cyril; Guijarro, J. I.; Chauvel, Murielle; Mourer, Thierry; Gow, Neil Ar; Aimanianda, Vishu Kumar; d'Enfert, Christophe; Fontaine, Thierry |
| Contributors: |
Biologie et Pathogénicité fongiques - Fungal Biology and Pathogenicity (BPF); Institut Pasteur Paris (IP)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE); Dynamic Microbiology - EA 7380 (DYNAMIC); École nationale vétérinaire d'Alfort (ENVA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est (UPE)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12); Plate-forme de bioimagerie ultrastructurale - Ultrastructural BioImaging Core Facility; Institut Pasteur Paris (IP)-Université Paris Cité (UPCité); Plateforme Technologique de RMN Biologique et HDX-MS - Biological NMR and HDX-MS Technological Platform; Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité); Virologie Moléculaire Avancée / Advanced Molecular Virology (VMA); University of Exeter; Immunobiologie d'Aspergillus - Immunobiology of Aspergillus; CB is the recipient of a PhD fellowship from the Laboratoire d’Excellence Integrative Biology of Emerging Infectious Diseases (ANR-10-LABX-62-IBEID). We acknowledge support from the French Government’s Investissement d’Avenir program (Laboratoire d’Excellence Integrative Biology of Emerging Infectious Diseases ANR-10-LABX-62-IBEID ) and Institut Pasteur from Paris. VA and TF are supported by the Agence Nationale de la Recherche ANR-21-CE17-0032-01, FUNPOLYVAC grant. NG acknowledges support of Wellcome Trust Investigator, Collaborative, Equipment, Strategic and Biomedical Resource awards (101873, 200208, 215599, 224323). NG also thanks the MRC (MR/M026663/2) and the MRC Centre for Medical Mycology (MR/N006364/2) for support. This study/research was funded by the National Institute for Health and Care Research (NIHR) Exeter Biomedical Research Centre (BRC). The 800-MHz NMR spectrometer of the Institut Pasteur was partially funded by the Région Ile de France (SESAME 2014 NMRCHR grant no. 4014526). CS is grateful for support for equipment from the French Government Programme Investissements d’Avenir France BioImaging (FBI, No. ANR-10-INSB-04-01).; ANR-21-CE17-0032,FUNPOLYVAC,Explorer les polysaccharides fongiques de la paroi cellulaire pour les immunothérapies(2021); ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010); ANR-10-INBS-0004,France-BioImaging,Développment d'une infrastructure française distribuée coordonnée(2010) |
| Source: |
EISSN: 2050-084X ; eLife ; https://pasteur.hal.science/pasteur-05271836 ; eLife, 2024, 13, ⟨10.7554/eLife.100569⟩ |
| Publisher Information: |
CCSD; eLife Sciences Publication |
| Publication Year: |
2024 |
| Subject Terms: |
[SDV]Life Sciences [q-bio] |
| Description: |
International audience ; The cell wall of human fungal pathogens plays critical roles as an architectural scaffold and as a target and modulator of the host immune response. Although the cell wall of the pathogenic yeast Candida albicans is intensively studied, one of the major fibrillar components in its cell wall, β-1,6-glucan, has been largely neglected. Here, we show that β-1,6-glucan is essential for bilayered cell wall organization, cell wall integrity, and filamentous growth. For the first time, we show that β-1,6-glucan production compensates the defect in mannan elongation in the outer layer of the cell wall. In addition, β-1,6-glucan dynamics are also coordinated by host environmental stimuli and stresses with wall remodeling, where the regulation of β-1,6-glucan structure and chain length is a crucial process. As we point out that β-1,6-glucan is exposed at the yeast surface and modulate immune response, β-1,6-glucan must be considered a key factor in host–pathogen interactions. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/39636210; PUBMED: 39636210; PUBMEDCENTRAL: PMC11620752 |
| DOI: |
10.7554/eLife.100569 |
| Availability: |
https://pasteur.hal.science/pasteur-05271836; https://pasteur.hal.science/pasteur-05271836v2/document; https://pasteur.hal.science/pasteur-05271836v2/file/elife-100569-v1.pdf; https://doi.org/10.7554/eLife.100569 |
| Rights: |
http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.7285B085 |
| Database: |
BASE |