| Title: |
Characterization of ESBL (SHV-12) producing clinical isolate of Enterobacter aerogenesfrom a tertiary care hospital in Nigeria |
| Authors: |
Kasap, Murat; Fashae, Kayode; Torol, Sinem; Kolayli, Fetiye; Budak, Fatma; Vahaboglu, Haluk |
| Publisher Information: |
BioMed Central Ltd. |
| Publication Year: |
2010 |
| Collection: |
BioMed Central |
| Description: |
Background We studied the beta-lactamases of an E. aerogenes isolate recovered from the blood of a two-year-old patient. The isolate demonstrated a disk-diffusion phenotype typical for an AmpC-ESBL co-producer. Methods Microbiology studies were performed according to standard protocols. The resistance gene was identified by transconjugation and cloning experiments. Results By transconjugation only a narrow spectrum beta-lactamase (TEM-1) encoded on a small plasmid was transmitted. The ESBL was cloned and expressed in an E. coli host. Sequence analysis of the recombinant plasmid revealed bla SHV-12 associated to the insertion sequence, IS 26 . Conclusion This is the first study demonstrated the occurrence of SHV-12 in Nigeria. |
| Document Type: |
report |
| Language: |
English |
| Relation: |
http://www.ann-clinmicrob.com/content/9/1/1 |
| Availability: |
http://www.ann-clinmicrob.com/content/9/1/1 |
| Rights: |
Copyright 2010 Kasap et al; licensee BioMed Central Ltd. |
| Accession Number: |
edsbas.7340891B |
| Database: |
BASE |