| Title: |
Alcohol‐associated intestinal dysbiosis alters mucosal‐associated invariant T‐cell phenotype and function |
| Authors: |
Gu, Min; Samuelson, Derrick R.; Taylor, Christopher M.; Molina, Patricia E.; Luo, Meng; Siggins, Robert W.; Shellito, Judd E.; Welsh, David A. |
| Contributors: |
National Institute on Alcohol Abuse and Alcoholism; National Institute of General Medical Sciences |
| Source: |
Alcoholism: Clinical and Experimental Research ; volume 45, issue 5, page 934-947 ; ISSN 0145-6008 1530-0277 |
| Publisher Information: |
Wiley |
| Publication Year: |
2021 |
| Collection: |
Wiley Online Library (Open Access Articles via Crossref) |
| Description: |
Background Chronic alcohol consumption is associated with a compromised innate and adaptive immune responses to infectious disease. Mucosa‐associated invariant T (MAIT) cells play a critical role in antibacterial host defense. However, whether alcohol‐associated deficits in innate and adaptive immune responses are mediated by alterations in MAIT cells remains unclear. Methods To investigate the impact of alcohol on MAIT cells, mice were treated with binge‐on‐chronic alcohol for 10 days and sacrificed at day 11. MAIT cells in the barrier organs (lung, liver, and intestine) were characterized by flow cytometry. Two additional sets of animals were used to examine the involvement of gut microbiota on alcohol‐induced MAIT cell changes: (1) Cecal microbiota from alcohol‐fed (AF) mice were adoptive transferred into antibiotic‐pretreated mice and (2) AF mice were treated with antibiotics during the experiment. MAIT cells in the barrier organs were measured via flow cytometry. Results Binge‐on‐chronic alcohol feeding led to a significant reduction in the abundance of MAIT cells in the barrier tissues. However, CD69 expression on tissue‐associated MAIT cells was increased in AF mice compared with pair‐fed (PF) mice. The expression of Th1 cytokines and the corresponding transcriptional factor was tissue specific, showing downregulation in the intestine and increases in the lung and liver in AF animals. Transplantation of fecal microbiota from AF mice resulted in a MAIT cell profile aligned to that of AF mouse donor. Antibiotic treatment abolished the MAIT cell differences between AF and PF animals. Conclusion MAIT cells in the intestine, liver, and lung are perturbed by alcohol use and these changes are partially attributable to alcohol‐associated dysbiosis. MAIT cell dysfunction may contribute to alcohol‐induced innate and adaptive immunity and consequently end‐organ pathophysiology. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1111/acer.14589 |
| Availability: |
https://doi.org/10.1111/acer.14589; https://onlinelibrary.wiley.com/doi/pdf/10.1111/acer.14589; https://onlinelibrary.wiley.com/doi/full-xml/10.1111/acer.14589 |
| Rights: |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
| Accession Number: |
edsbas.74170170 |
| Database: |
BASE |