Variation in the measurement of DNA damage by comet assay measured by the ECVAG inter-laboratory validation trial
| Title: | Variation in the measurement of DNA damage by comet assay measured by the ECVAG inter-laboratory validation trial |
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| Authors: | Forchhammer, Lykke; Johansson, Clara; Loft, Steffen; Möller, Lennart; Godschalk, Roger W L; Langie, Sabine A S; Jones, George D D; Kwok, Rachel W L; Collins, Andrew R; Azqueta, Amaya; Phillips, David H; Sozeri, Osman; Stepnik, Maciej; Palus, Jadwiga; Vogel, Ulla; Wallin, Håkan; Routledge, Michael N; Handforth, Catherine; Allione, Alessandra; Matullo, Giuseppe; Teixeira, João Paulo; Costa, Solange; Riso, Patrizia; Porrini, Marisa; Møller, Peter |
| Source: | Forchhammer, L, Johansson, C, Loft, S, Möller, L, Godschalk, R W L, Langie, S A S, Jones, G D D, Kwok, R W L, Collins, A R, Azqueta, A, Phillips, D H, Sozeri, O, Stepnik, M, Palus, J, Vogel, U, Wallin, H, Routledge, M N, Handforth, C, Allione, A, Matullo, G, Teixeira, J P, Costa, S, Riso, P, Porrini, M & Møller, P 2010, 'Variation in the measurement of DNA damage by comet assay measured by the ECVAG inter-laboratory validation trial', Mutagenesis, vol. 25, no. 2, pp. 113 - 123. https://doi.org/10.1093/mutage/gep048 |
| Publication Year: | 2010 |
| Collection: | King's College, London: Research Portal |
| Subject Terms: | Calibration; Cells; Cultured; Comet Assay; Cryopreservation; DNA Damage; DNA-Formamidopyrimidine Glycosylase; Humans; Laboratories; Monocytes; Observer Variation; Radiation; Ionizing; Reference Standards |
| Description: | The comet assay has become a popular method for the assessment of DNA damage in biomonitoring studies and genetic toxicology. However, few studies have addressed the issue of the noted inter-laboratory variability of DNA damage measured by the comet assay. In this study, 12 laboratories analysed the level of DNA damage in monocyte-derived THP-1 cells by either visual classification or computer-aided image analysis of pre-made slides, coded cryopreserved samples of cells and reference standard cells (calibration curve samples). The reference standard samples were irradiated with ionizing radiation (0-10 Gy) and used to construct a calibration curve to calculate the number of lesions per 10(6) base pair. All laboratories detected dose-response relationships in the coded samples irradiated with ionizing radiation (1.5-7 Gy), but there were overt differences in the level of DNA damage reported by the different laboratories as evidenced by an inter-laboratory coefficient of variation (CV) of 47%. Adjustment of the primary comet assay end points by a calibration curve prepared in each laboratory reduced the CV to 28%, a statistically significant reduction (P |
| Document Type: | article in journal/newspaper |
| Language: | English |
| ISSN: | 1464-3804 |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/19910383; info:eu-repo/semantics/altIdentifier/wos/000274986100003; info:eu-repo/semantics/altIdentifier/eissn/1464-3804 |
| DOI: | 10.1093/mutage/gep048 |
| Availability: | https://kclpure.kcl.ac.uk/portal/en/publications/bd40e81b-f8b7-4170-9e48-f3cdfa738bae; https://doi.org/10.1093/mutage/gep048 |
| Rights: | info:eu-repo/semantics/openAccess |
| Accession Number: | edsbas.74E86F97 |
| Database: | BASE |