| Title: |
Quinazoline-Derivatives of Imino-1,2,3-Dithiazoles Promote Biofilm Dispersion of Pseudomonas aeruginosa |
| Authors: |
Gonzalez, Mathieu; Tareau, Anne-Sophie; de Crozals, Daphnée; Layec, Corentin; Broudic, Nathan; Barreau, Magalie; Forge, Adrien; Lesouhaitier, Olivier; Fruit, Corinne; Chevalier, Sylvie; Besson, Thierry; Tahrioui, Ali |
| Contributors: |
Communication Bactérienne et Stratégies Anti-infectieuses (CBSA); Université de Caen Normandie (UNICAEN); Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN); Normandie Université (NU); Chimie Analytique et Réactivité Moléculaire En Normandie (Institut CARMeN); Normandie Université (NU)-Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN); Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN); Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie); Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS); This work was partially supported by Normandie Univ., the Région Normandie, the Centre National de la Recherche Scientifique, Univ. de Rouen Normandie, INSA Rouen Normandie, Univ. Caen Normandie, ENSICAEN, Labex SynOrg (ANR-11-LABX-0029), the graduate school for research XL-Chem (ANR-18-EURE-0020 XL CHEM), and Innovation Chimie Carnot (I2C).; ANR-11-LABX-0029,SYNORG,Synthèse Organique : des molécules au vivant(2011); ANR-18-EURE-0020,XL Chem,XLChem, Synthesizing our future(2018) |
| Source: |
ISSN: 1424-8247 ; Pharmaceuticals ; https://hal.science/hal-05469128 ; Pharmaceuticals, 2025, 18 (11), pp.1733. ⟨10.3390/ph18111733⟩. |
| Publisher Information: |
CCSD; MDPI |
| Publication Year: |
2025 |
| Collection: |
Normandie Université: HAL |
| Subject Terms: |
Pseudomonas aeruginosa; N-arylimino-1.2.3-dithiazoles; biofilm dispersion; [SDV]Life Sciences [q-bio] |
| Description: |
International audience ; Background/Objectives: Biofilm-associated infections pose a major clinical challenge since bacteria within biofilms exhibit highly antibiotic tolerance. Pseudomonas aeruginosa forms persistent biofilms that cause chronic infections in vulnerable patients, including those with cystic fibrosis, burns, or medical implants. Such biofilm-associated chronic infections require prolonged treatments that promote antimicrobial resistance. To address this, recent strategies focus on enhancing biofilm dispersion. Methods: Thirty-six N-arylimino-1,2,3-dithiazoles were screened for their biofilm dispersal activity using a crystal violet assay. Their cytotoxicity was assessed on A549 and HaCat eukaryotic cells. Moreover, their influence on bacterial growth and virulence was investigated. Lastly, fluorescence anisotropy was used to measure membrane fluidity to obtain the first insights on the mechanism of action of these chemicals. Results: Our results showed that quinazoline-derivatives of imino-1,2,3-dithiazoles display biofilm dispersion activity. These compounds do not increase virulence through pyocyanin production, do not modify the growth kinetics of P. aeruginosa, and do not show cytotoxicity towards eucaryotic cells. Conclusions: These findings highlight the potential use of N-arylimino-1,2,3-dithiazole-derived compounds as safe and effective dispersal agents of P. aeruginosa biofilms. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/41304977; PUBMED: 41304977; PUBMEDCENTRAL: PMC12655343 |
| DOI: |
10.3390/ph18111733 |
| Availability: |
https://hal.science/hal-05469128; https://hal.science/hal-05469128v1/document; https://hal.science/hal-05469128v1/file/pharmaceuticals-18-01733-v2.pdf; https://doi.org/10.3390/ph18111733 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.75227665 |
| Database: |
BASE |