| Title: |
A Single-Stranded DNA-Encoded Chemical Library Based on a Stereoisomeric Scaffold Enables Ligand Discovery by Modular Assembly of Building Blocks |
| Authors: |
Bassi, Gabriele; Favalli, Nicholas; Vuk, Miriam; Catalano, Marco; Martinelli, Adriano; Trenner, Anika; Porro, Antonio; Yang, Su; Tham, Chuin Lean; Moroglu, Mustafa; Yue, Wyatt W; Conway, Stuart J; Vogt, Peter K; Sartori, Alessandro A; Scheuermann, Jörg; Neri, Dario |
| Source: |
Bassi, Gabriele; Favalli, Nicholas; Vuk, Miriam; Catalano, Marco; Martinelli, Adriano; Trenner, Anika; Porro, Antonio; Yang, Su; Tham, Chuin Lean; Moroglu, Mustafa; Yue, Wyatt W; Conway, Stuart J; Vogt, Peter K; Sartori, Alessandro A; Scheuermann, Jörg; Neri, Dario (2020). A Single-Stranded DNA-Encoded Chemical Library Based on a Stereoisomeric Scaffold Enables Ligand Discovery by Modular Assembly of Building Blocks. Advanced Science, 7(22):2001970. |
| Publisher Information: |
Wiley Open Access |
| Publication Year: |
2020 |
| Collection: |
University of Zurich (UZH): ZORA (Zurich Open Repository and Archive |
| Subject Terms: |
Institute of Molecular Cancer Research; 570 Life sciences; biology; 610 Medicine & health |
| Description: |
A versatile and Lipinski-compliant DNA-encoded library (DEL), comprising 366 600 glutamic acid derivatives coupled to oligonucleotides serving as amplifiable identification barcodes is designed, constructed, and characterized. The GB-DEL library, constructed in single-stranded DNA format, allows de novo identification of specific binders against several pharmaceutically relevant proteins. Moreover, hybridization of the single-stranded DEL with a set of known protein ligands of low to medium affinity coupled to a complementary DNA strand results in self-assembled selectable chemical structures, leading to the identification of affinity-matured compounds. |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
English |
| ISSN: |
2198-3844 |
| Relation: |
https://www.zora.uzh.ch/id/eprint/199547/1/Bassi_AdvSci_2020.pdf; info:pmid/33240760; urn:issn:2198-3844 |
| DOI: |
10.1002/advs.202001970 |
| Availability: |
https://www.zora.uzh.ch/id/eprint/199547/; https://www.zora.uzh.ch/id/eprint/199547/1/Bassi_AdvSci_2020.pdf; https://doi.org/10.1002/advs.202001970 |
| Rights: |
info:eu-repo/semantics/openAccess ; Creative Commons: Attribution 4.0 International (CC BY 4.0) ; http://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.76020BA3 |
| Database: |
BASE |