Sensitivity of SARS-CoV-2 RNA polymerase chain reaction using a clinical and radiological reference standard: Clinical sensitivity of SARS-CoV-2 PCR.
| Title: | Sensitivity of SARS-CoV-2 RNA polymerase chain reaction using a clinical and radiological reference standard: Clinical sensitivity of SARS-CoV-2 PCR. |
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| Authors: | Stockdale, Alexander J; Fyles, Fred; Farrell, Catriona; Lewis, Joe; Barr, David; Haigh, Kathryn; Abouyannis, Michael; Hankinson, Beth; Penha, Diana; Fernando, Rashika; Wiles, Rebecca; Sharma, Sheetal; Santamaria, Nuria; Chindambaram, Vijay; Probert, Cairine; Ahmed, Muhammad Shamsher; Cruise, James; Fordham, Imogen; Hicks, Rory; Maxwell, Alice; Moody, Nick; Paterson, Tamsin; Stott, Katharine; Wu, Meng-San; Beadsworth, Michael; Todd, Stacy; Joekes, Elizabeth |
| Publisher Information: | Elsevier BV |
| Publication Year: | 2021 |
| Collection: | The University of Liverpool Repository |
| Description: | Objectives Diagnostic tests for SARS-CoV-2 are important for epidemiology, clinical management, and infection control. Limitations of oro-nasopharyngeal real-time PCR sensitivity have been described based on comparisons of single tests with repeated sampling. We assessed SARS-CoV-2 PCR clinical sensitivity using a clinical and radiological reference standard. Methods Between March-May 2020, 2060 patients underwent thoracic imaging and SARS-CoV-2 PCR testing. Imaging was independently double- or triple-reported (if discordance) by blinded radiologists according to radiological criteria for COVID-19. We excluded asymptomatic patients and those with alternative diagnoses that could explain imaging findings. Associations with PCR-positivity were assessed with binomial logistic regression. Results 901 patients had possible/probable imaging features and clinical symptoms of COVID-19 and 429 patients met the clinical and radiological reference case definition. SARS-CoV-2 PCR sensitivity was 68% (95% confidence interval 64-73), was highest 7-8 days after symptom onset (78% (68-88)) and was lower among current smokers (adjusted odds ratio 0.23 (0.12-0.42) p |
| Document Type: | article in journal/newspaper |
| File Description: | text |
| Language: | English |
| ISSN: | 0163-4453 |
| Relation: | https://livrepository.liverpool.ac.uk/3120856/1/COVID-19%20diagnostic%20performance_J%20Infection_author%20accepted.docx; Collapse authors list. Stockdale, Alexander J orcid:0000-0002-5828-3328 , Fyles, Fred orcid:0000-0002-5141-6220 , Farrell, Catriona, Lewis, Joe orcid:0000-0002-3837-5188 , Barr, David orcid:0000-0002-2922-9381 , Haigh, Kathryn orcid:0000-0003-1901-7315 , Abouyannis, Michael, Hankinson, Beth, Penha, Diana, Fernando, Rashika et al (show 17 more authors) , Wiles, Rebecca, Sharma, Sheetal, Santamaria, Nuria, Chindambaram, Vijay, Probert, Cairine, Ahmed, Muhammad Shamsher orcid:0000-0001-6163-8102 , Cruise, James, Fordham, Imogen, Hicks, Rory, Maxwell, Alice, Moody, Nick, Paterson, Tamsin, Stott, Katharine orcid:0000-0001-7079-7957 , Wu, Meng-San, Beadsworth, Michael, Todd, Stacy and Joekes, Elizabeth (2021) Sensitivity of SARS-CoV-2 RNA polymerase chain reaction using a clinical and radiological reference standard: Clinical sensitivity of SARS-CoV-2 PCR. The Journal of infection, 82 (6). pp. 260-268. ISSN 0163-4453, 1532-2742 |
| DOI: | 10.1016/j.jinf.2021.04.012 |
| Availability: | https://livrepository.liverpool.ac.uk/3120856/; https://doi.org/10.1016/j.jinf.2021.04.012 |
| Accession Number: | edsbas.76233076 |
| Database: | BASE |