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Nocathioamides, Uncovered by a Tunable Metabologenomic Approach, Define a Novel Class of Chimeric Lanthipeptides

Title: Nocathioamides, Uncovered by a Tunable Metabologenomic Approach, Define a Novel Class of Chimeric Lanthipeptides
Authors: Saad, Hamada; Aziz, Saefuddin; Gehringer, Matthias; Kramer, Markus; Straetener, Jan; Berscheid, Anne; Brötz‐Oesterhelt, Heike; Gross, Harald
Contributors: Deutsches Zentrum für Infektionsforschung; Deutsche Forschungsgemeinschaft; Ministry of Higher Education
Source: Angewandte Chemie International Edition ; volume 60, issue 30, page 16472-16479 ; ISSN 1433-7851 1521-3773
Publisher Information: Wiley
Publication Year: 2021
Collection: Wiley Online Library (Open Access Articles via Crossref)
Description: The increasing number of available genomes, in combination with advanced genome mining techniques, unveiled a plethora of biosynthetic gene clusters (BGCs) coding for ribosomally synthesized and post‐translationally modified peptides (RiPPs). The products of these BGCs often represent an enormous resource for new and bioactive compounds, but frequently, they cannot be readily isolated and remain cryptic. Here, we describe a tunable metabologenomic approach that recruits a synergism of bioinformatics in tandem with isotope‐ and NMR‐guided platform to identify the product of an orphan RiPP gene cluster in the genomes of Nocardia terpenica IFM 0406 and 0706 T . The application of this tactic resulted in the discovery of nocathioamides family as a founder of a new class of chimeric lanthipeptides I.
Document Type: article in journal/newspaper
Language: English
DOI: 10.1002/anie.202102571
Availability: https://doi.org/10.1002/anie.202102571; https://onlinelibrary.wiley.com/doi/pdf/10.1002/anie.202102571; https://onlinelibrary.wiley.com/doi/full-xml/10.1002/anie.202102571
Rights: http://creativecommons.org/licenses/by-nc-nd/4.0/
Accession Number: edsbas.76A199B4
Database: BASE