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A Peculiar CLL Case with Complex Chromosome 6 Rearrangements and Refinement of All Breakpoints at the Gene Level by Genomic Array: A Case Report

Title: A Peculiar CLL Case with Complex Chromosome 6 Rearrangements and Refinement of All Breakpoints at the Gene Level by Genomic Array: A Case Report
Authors: Michele Cennamo; Davide Sirocchi; Carolina Giudici; Marzia Giagnacovo; Guido Petracco; Daniela Ferrario; Simona Garganigo; Angela Papa; Emanuela Veniani; Alessandro Squizzato; Lucia Del Vecchio; Carlo Patriarca; Michelarcangelo Partenope; Piergiorgio Modena
Source: Journal of Clinical Medicine, Vol 12, Iss 12, p 4110 (2023)
Publisher Information: MDPI AG
Publication Year: 2023
Collection: Directory of Open Access Journals: DOAJ Articles
Subject Terms: chronic lymphocytic leukemia; CLL; autoimmune hemolytic anemia; AIHA; complex karyotype; FISH; Medicine
Description: Introduction: Chronic lymphocytic leukemia (CLL), the most common leukemia in Western countries, is a mature B-cell chronic lymphoproliferative disorder characterized by the accumulation of neoplastic CD5+ B lymphocytes, functionally incompetent and usually monoclonal in origin, in bone marrow, lymph nodes and blood. Diagnosis occurs predominantly in elderly patients, with a median age reported between 67 and 72 years. CLL has a heterogeneous clinical course, which can vary from indolent to, less frequently, aggressive forms. Early-stage asymptomatic CLL patients do not require immediate therapeutic intervention, but only observation; treatment is necessary for patients with advanced disease or when “active disease” is observed. The most frequent autoimmune cytopenia (AIC) is autoimmune haemolytic anaemia (AHIA). The main mechanisms underlying the appearance of AIC in CLL are not fully elucidated, the predisposition of patients with CLL to suffering autoimmune complications is variable and autoimmune cytopenia can precede, be concurrent, or follow the diagnosis of CLL. Case presentation: A 74-year-old man was admitted to the emergency room following the finding of severe macrocytic anaemia during blood tests performed that same day, in particular the patient showed a profound asthenia dating back several months. The anamnesis was silent and the patient was not taking any medications. The blood examination showed an extremely high White Blood Cell count and findings of AIHA in CLL-type mature B-cell lymphoproliferative neoplasia. Genetic investigations: Conventional karyotyping was performed and it obtained a trisomy 8 and an unbalanced translocation between the short arm of chromosome 6 and the long arm of chromosome 11, concurrent with interstitial deletions in chromosomes 6q and 11q that could not be defined in detail. Molecular cytogenetics (FISH) analyses revealed Ataxia Telangiectasia Mutated (ATM) monoallelic deletion (with loss of ATM on derivative chromosome 11) and retained signals for TP53, 13q14 and ...
Document Type: article in journal/newspaper
Language: English
Relation: https://www.mdpi.com/2077-0383/12/12/4110; https://doaj.org/toc/2077-0383; https://doaj.org/article/92fff451bc14479bab463bb4964d9165
DOI: 10.3390/jcm12124110
Availability: https://doi.org/10.3390/jcm12124110; https://doaj.org/article/92fff451bc14479bab463bb4964d9165
Accession Number: edsbas.7822E786
Database: BASE