| Title: |
Urethroplasty with a bilayered poly-D,L-lactide-co-ε-caprolactone scaffold seeded with allogenic mesenchymal stem cells. |
| Authors: |
Yudintceva, Natalia M; Nashchekina, Yulia A; Mikhailova, Nataliya A; Vinogradova, Tatiana I; Yablonsky, Petr K; Gorelova, Anna A; Muraviov, Alexandr N; Gorelov, Andrey V; Samusenko, Igor A; Nikolaev, Boris P; Yakovleva, Ludmila Y; Shevtsov, Maxim A |
| Contributors: |
Klinik und Poliklinik für RadioOnkologie und Strahlentherapie |
| Publication Year: |
2020 |
| Collection: |
Munich University of Technology (TUM): mediaTUM |
| Subject Terms: |
info:eu-repo/classification/ddc |
| Description: |
Reconstructive surgery for urethral defects employing tissue-engineered scaffolds represents an alternative treatment for urethroplasty. The aim of this study was to compare the therapeutic efficacy of the bilayer poly-D,L-lactide/poly-ε-caprolactone (PL-PC) scaffold seeded with allogenic mesenchymal stem cells (MSCs) for urethra reconstruction in a rabbit model with conventional urethroplasty employing an autologous buccal mucosa graft (BG). The inner layer of the scaffold based on poly-D,L-lactic acid (PL) was seeded with MSCs, while the outer layer, prepared from poly-ε-caprolactone, protected the surrounding tissues from urine. To track the MSCs in vivo, the latter were labeled with superparamagnetic iron oxide nanoparticles. In rabbits, a dorsal penile defect was reconstructed employing a BG or a PL-PC graft seeded with nanoparticle-labeled MSCs. In the 12-week follow-up period, no complications were detected. Subsequent histological analysis demonstrated biointegration of the PL-PC graft with surrounding urethral tissues. Less fibrosis and inflammatory cell infiltration were observed in the experimental group as compared with the BG group. Nanoparticle-labeled MSCs were detected in the urothelium and muscular layer, co-localizing with the urothelium cytokeratin marker AE1/AE3, indicating the possibility of MSC differentiation into neo-urothelium. Our results suggest that a bilayer MSCs-seeded scaffold could be efficiently employed for urethroplasty. |
| Document Type: |
article in journal/newspaper |
| Language: |
unknown |
| Relation: |
https://mediatum.ub.tum.de/1591151 |
| DOI: |
10.1002/jbm.b.34453 |
| Availability: |
https://mediatum.ub.tum.de/1591151; https://doi.org/10.1002/jbm.b.34453 |
| Rights: |
info:eu-repo/semantics/restrictedAccess |
| Accession Number: |
edsbas.788EC2FF |
| Database: |
BASE |