| Title: |
Shedding Light on Metal‐Based Nanoparticles in Zebrafish by Computed Tomography with Micrometer Resolution |
| Authors: |
Cörek, Emre; Rodgers, Griffin; Siegrist, Stefan; Einfalt, Tomaz; Detampel, Pascal; Schlepütz, Christian M.; Sieber, Sandro; Fluder, Pascal; Schulz, Georg; Unterweger, Harald; Alexiou, Christoph; Müller, Bert; Puchkov, Maxim; Huwyler, Jörg |
| Contributors: |
Swiss Centre for Applied Human Toxicology; Horizon 2020 |
| Source: |
Small ; volume 16, issue 31 ; ISSN 1613-6810 1613-6829 |
| Publisher Information: |
Wiley |
| Publication Year: |
2020 |
| Collection: |
Wiley Online Library (Open Access Articles via Crossref) |
| Description: |
Metal‐based nanoparticles are clinically used for diagnostic and therapeutic applications. After parenteral administration, they will distribute throughout different organs. Quantification of their distribution within tissues in the 3D space, however, remains a challenge owing to the small particle diameter. In this study, synchrotron radiation‐based hard X‐ray tomography (SRμCT) in absorption and phase contrast modes is evaluated for the localization of superparamagnetic iron oxide nanoparticles (SPIONs) in soft tissues based on their electron density and X‐ray attenuation. Biodistribution of SPIONs is studied using zebrafish embryos as a vertebrate screening model. This label‐free approach gives rise to an isotropic, 3D, direct space visualization of the entire 2.5 mm‐long animal with a spatial resolution of around 2 µm. High resolution image stacks are available on a dedicated internet page ( http://zebrafish.pharma-te.ch ). X‐ray tomography is combined with physico‐chemical characterization and cellular uptake studies to confirm the safety and effectiveness of protective SPION coatings. It is demonstrated that SRμCT provides unprecedented insights into the zebrafish embryo anatomy and tissue distribution of label‐free metal oxide nanoparticles. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1002/smll.202000746 |
| Availability: |
https://doi.org/10.1002/smll.202000746; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fsmll.202000746; https://onlinelibrary.wiley.com/doi/pdf/10.1002/smll.202000746; https://onlinelibrary.wiley.com/doi/full-xml/10.1002/smll.202000746 |
| Rights: |
http://creativecommons.org/licenses/by-nc/4.0/ |
| Accession Number: |
edsbas.78C14D0E |
| Database: |
BASE |