| Title: |
The BMP7-Derived Peptide p[63-82] Reduces Cartilage Degeneration in the Rat ACLT-pMMx Model for Posttraumatic Osteoarthritis |
| Authors: |
Ripmeester, Ellen G. J.; Steijns, Jessica S. J. J.; Wijnands, Karolina A. P.; Stassen, Roderick H. M. J.; Pitelka, Vasek; Peeters, Laura C. W.; Cremers, Andy; Astryde, Nzekui M. S. A.; Chabronova, Alzbeta; Surtel, Don A. M.; Emans, Pieter J.; van den Akker, Guus G. H.; van Rietbergen, Bert; van Rhijn, Lodewijk W.; Caron, Marjolein M. J.; Welting, Tim J. M. |
| Source: |
Ripmeester, E G J, Steijns, J S J J, Wijnands, K A P, Stassen, R H M J, Pitelka, V, Peeters, L C W, Cremers, A, Astryde, N M S A, Chabronova, A, Surtel, D A M, Emans, P J, van den Akker, G G H, van Rietbergen, B, van Rhijn, L W, Caron, M M J & Welting, T J M 2025, 'The BMP7-Derived Peptide p[63-82] Reduces Cartilage Degeneration in the Rat ACLT-pMMx Model for Posttraumatic Osteoarthritis', Cartilage, vol. 16, no. 4, pp. 495-506. https://doi.org/10.1177/19476035241233659 |
| Publication Year: |
2025 |
| Collection: |
Maastricht University Research Publications |
| Subject Terms: |
articular cartilage; osteoarthritis; anterior cruciate ligament tear-partial meniscectomy; peptides; subchondral bone; cartilage degeneration; pain; ARTICULAR-CARTILAGE; WEIGHT-BEARING; SYNOVITIS; PATHOGENESIS; EFFICACY; DISEASE |
| Description: |
Objective: Osteoarthritis (OA) is characterized by articular cartilage erosion, pathological subchondral bone changes, and signs of synovial inflammation and pain. We previously identified p[63-82], a bone morphogenetic protein 7 (BMP7)-derived bioactive peptide that attenuates structural cartilage degeneration in the rat medial meniscal tear-model for posttraumatic OA. This study aimed to evaluate the cartilage erosion-attenuating activity of p[63-82] in a different preclinical model for OA (anterior cruciate ligament transection—partial medial meniscectomy [anterior cruciate ligament transection (ACLT)-pMMx]). The disease-modifying action of the p[63-82] was followed-up in this model for 5 and 10 weeks. Design: Skeletally mature male Lewis rats underwent ACLT-pMMx surgery. Rats received weekly intra-articular injections with either saline or 500 ng p[63-82]. Five and 10 weeks postsurgery, rats were sacrificed, and subchondral bone characteristics were determined using microcomputed tomography (µCT). Histopathological evaluation of cartilage degradation and Osteoarthritis Research Society International (OARSI)-scoring was performed following Safranin-O/Fast Green staining. Pain-related behavior was measured by incapacitance testing and footprint analysis. Results: Histopathological evaluation at 5 and 10 weeks postsurgery showed reduced cartilage degeneration and a significantly reduced OARSI score, whereas no significant changes in subchondral bone characteristics were found in the p[63-82]-treated rats compared to the saline-treated rats. ACLT-pMMx-induced imbalance of static weightbearing capacity in the p[63-82] group was significantly improved compared to the saline-treated rats at weeks 5 postsurgery. Footprint analysis scores in the p[63-82]-treated rats demonstrated improvement at week 10 postsurgery. Conclusions: Weekly intra-articular injections of p[63-82] in the rat ACLT-pMMx posttraumatic OA model resulted in reduced degenerative cartilage changes and induced functional improvement in static ... |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| ISSN: |
1947-6035; 1947-6043 |
| Relation: |
info:eu-repo/semantics/altIdentifier/wos/001187830300001; info:eu-repo/semantics/altIdentifier/pissn/1947-6035; info:eu-repo/semantics/altIdentifier/eissn/1947-6043 |
| DOI: |
10.1177/19476035241233659 |
| Availability: |
https://cris.maastrichtuniversity.nl/en/publications/f004b5a0-22f6-4f75-b064-59ab9cf52b17; https://doi.org/10.1177/19476035241233659 |
| Rights: |
info:eu-repo/semantics/openAccess ; http://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.790BFD25 |
| Database: |
BASE |