| Title: |
Treatment of protoscoleces with gamma radiation: Potential immunoprotective effect against experimental murine echinococcosis |
| Authors: |
Hadj Rabia, Samia; Debib, Aicha; Mezaguer, Meriem; Yefsah, Rabah; Soufli, Imene; Hamrioui, Bousaad; Touil-Boukoffa, Chafia; Baz, Ahsene; Giaimis, Jean; Mameri, Saâdia |
| Contributors: |
Centre de Recherche Nucléaire de Draria (CRND); École normale supérieure - Kouba-Alger (ENS Kouba-Alger); Centre Universitaire Morsli Abdellah de Tipaza; Centre de Recherche Nucléaire d'Alger (CRNA); COMENA; Laboratoire de Biologie Cellulaire et Moléculaire; Université des Sciences et de la Technologie Houari Boumediene = University of Sciences and Technology Houari Boumediene Alger (USTHB); Hôpital Mustapha Pacha Alger (CHUMA); Démarche intégrée pour l'obtention d'aliments de qualité (UMR QualiSud); Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Avignon Université (AU)-Université de La Réunion (UR)-Institut Agro Montpellier; Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM); Directorate-General for Scientific Research and Technological Development (DGRSDT) of the Algerian Ministry of Higher Education and Scientific Research |
| Source: |
ISSN: 0141-9838. |
| Publisher Information: |
CCSD; Wiley |
| Publication Year: |
2022 |
| Collection: |
Université de Montpellier: HAL |
| Subject Terms: |
cystic echinococcosis; cytokines; histological analysis; immunization; immunoprotection; radio-attenuated protoscoleces; MESH: Animals; MESH: Cysts; MESH: Cytokines; MESH: Echinococcosis; MESH: Echinococcus; MESH: Echinococcus granulosus; MESH: Gamma Rays; MESH: Larva; MESH: Mice; [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health; [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology |
| Description: |
International audience ; Cystic echinococcosis (CE) is one of the most important zoonotic diseases with a worldwide distribution. It is caused by the larval stage of the dog tapeworm “ Echinococcus granulosussensu lato ” and constitutes a major economic and public health problem in several countries. Protoscoleces are one component of this larval stage that can interact with both definitive and intermediate hosts. The aim of the present study was to investigate the potential role of using a radio‐attenuated form of these protoscoleces for immunoprophylaxis against experimental murine echinococcosis. However, mice were immunized twice at 15‐day intervals with gamma (γ) irradiated protoscoleces at doses of 0.4, 0.8, 1.2 and 1.4 kGy then challenged with the intact parasites. Macroscopic and histological analyses with cytokine measurements were performed in order to estimate the number and diameter of cysts, microscopic changes and cytokine profile. An improvement in protection against the challenge dose was observed with increasing dose, giving percentages of 47.7, 49, 55.23 and 70.6%, for the 0.4, 0.8, 1.2 and 1.4 kGy‐groups respectively. These data suggest that immunization with radio‐attenuated protoscoleces may induce satisfactory protective immunity by reducing successfully the formation of cysts, caused by challenge infection. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/36054717; PUBMED: 36054717 |
| DOI: |
10.1111/pim.12944 |
| Availability: |
https://hal.science/hal-05449942; https://doi.org/10.1111/pim.12944 |
| Accession Number: |
edsbas.79414391 |
| Database: |
BASE |