| Title: |
Host Genetic Variation Does Not Determine Spatio-Temporal Patterns of European Bat 1 Lyssavirus |
| Authors: |
Troupin, Cécile; Picard-Meyer, Evelyne; Dellicour, Simon; Casademont, Isabelle; Kergoat, Lauriane; Lepelletier, Anthony; Dacheux, Laurent; Baele, Guy; Monchâtre-Leroy, Elodie; Cliquet, Florence; Lemey, Philippe; Bourhy, Hervé |
| Contributors: |
Centre Collaborateur de l'OMS pour la Rage - Dynamique des lyssavirus et adaptation à l'hôte (CC-OMS); Institut Pasteur Paris (IP); Laboratoire de la rage et de la faune sauvage de Nancy (LRFSN); Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES); Rega Institute for Medical Research Leuven; Catholic University of Leuven = Katholieke Universiteit Leuven (KU Leuven); Génétique fonctionnelle des maladies infectieuses - Functional Genetics of Infectious Diseases; Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS); This work was supported by European Union Seventh Framework Programme (FP7/2007-2013) PREDEMICS (under grant agreement number 278433), Santé Publique France and the Institut Pasteur. SD is funded by the Fonds Wetenschappelijk Onderzoek (FWO, Flanders, Belgium). PL acknowledges the Research Foundation - Flanders (G0D5117N & G066215) and the Research Fund KU Leuven (OT/14/115).; The author want to acknowledge Karin Eiglmeier from Unit of Insect Vector Genetics and Genomics for access and help on ABI Prism 3730 for microsatellite genotyping. We would like to thank the SFEPM group (Societe Francaise pour l’Etude et la Protection des Mammiferes, groupe Chiropteres), and especially the bat naturalists, for their collaboration in the surveillance of bat rabies in France.; European Project: 278433,FP7-HEALTH-2011-two-stage,FP7-HEALTH-2011-two-stage,PREDEMICS(2011) |
| Source: |
ISSN: 1759-6653. |
| Publisher Information: |
CCSD; Society for Molecular Biology and Evolution |
| Publication Year: |
2017 |
| Collection: |
Institut Pasteur: HAL |
| Subject Terms: |
genome sequence; European bat 1 lyssavirus (EBLV-1); RNA virus evolution; serotine bat; next generation sequencing; MESH: Animals; MESH: Biological Evolution; MESH: Selection; Genetic; MESH: Cell Nucleus; MESH: Chiroptera; MESH: DNA; Mitochondrial; MESH: Europe; MESH: Genome; Viral; MESH: Host-Pathogen Interactions; MESH: Lyssavirus; MESH: Microsatellite Repeats; [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology |
| Description: |
International audience ; The majority of bat rabies cases in Europe are attributed to European bat 1 lyssavirus (EBLV-1), circulating mainly in serotine bats (Eptesicus serotinus). Two subtypes have been defined (EBLV-1a and EBLV-1b), each associated with a different geographical distribution. In this study, we undertake a comprehensive sequence analysis based on 80 newly obtained EBLV-1 nearly complete genome sequences from nine European countries over a 45-year period to infer selection pressures, rates of nucleotide substitution, and evolutionary time scale of these two subtypes in Europe. Our results suggest that the current lineage of EBLV-1 arose in Europe $600 years ago and the virus has evolved at an estimated average substitution rate of $4.19Â10 À5 subs/site/ year, which is among the lowest recorded for RNA viruses. In parallel, we investigate the genetic structure of French serotine bats at both the nuclear and mitochondrial level and find that they constitute a single genetic cluster. Furthermore, Mantel tests based on interindividual distances reveal the absence of correlation between genetic distances estimated between viruses and between host individuals. Taken together, this indicates that the genetic diversity observed in our E. serotinus samples does not account for EBLV-1a and-1b segregation and dispersal in Europe. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/29165566; info:eu-repo/grantAgreement//278433/EU/Preparedness, Prediction and Prevention of Emerging Zoonotic Viruses with Pandemic Potential using Multidisciplinary Approaches/PREDEMICS; PUBMED: 29165566; PUBMEDCENTRAL: PMC5721339 |
| DOI: |
10.1093/gbe/evx236 |
| Availability: |
https://pasteur.hal.science/pasteur-03112525; https://pasteur.hal.science/pasteur-03112525v1/document; https://pasteur.hal.science/pasteur-03112525v1/file/evx236.pdf; https://doi.org/10.1093/gbe/evx236 |
| Rights: |
http://creativecommons.org/licenses/by-nc/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.7A973A1C |
| Database: |
BASE |