| Title: |
Local and transboundary transmission of methicillin-resistant Staphylococcus aureus sequence type 398 through pig trading |
| Authors: |
Pirolo, M; Sieber, R N; Moodley, A; Visaggio, D; Artuso, I; Gioffrè, A; Casalinuovo, F; Spatari, G; Guardabassi, L; Stegger, M; Visca, P |
| Publication Year: |
2020 |
| Collection: |
RVC Research Online (Royal Veterinary College, University of London) |
| Description: |
Livestock-associated methicillin-resistant Staphylococcus aureus sequence type (ST) 398 (LA-MRSA ST398) is a genetic lineage for which pigs are regarded as the main reservoir. An increasing prevalence of LA-MRSA ST398 has been reported in areas with high livestock density throughout Europe. In this study, we have investigated the drivers contributing to the introduction and spread of LA-MRSA ST398 along the pig farming system in Southern Italy. Whole-genome sequencing (WGS) of LA-MRSA ST398 isolates collected in 2018 from pigs (n=53) and employees (n=14) from 10 farms in the Calabria region were comparatively analysed with previously published WGS data from Italian ST398 isolates (n=45), an international ST398 reference collection (n=89) and isolates from Danish pigs farms (n=283), which are the main suppliers of pigs imported to Italy. Single-nucleotide polymorphisms (SNP) were used to infer isolates relatedness and, together with data from animal trading, factors contributing to LA-MRSA ST398 dissemination were identified. The analyses support the existence of two concurrent pathways for the spread of LA-MRSA ST398 in Southern Italy: i) multiple introductions of LA-MRSA ST398 through the import of colonized pigs from other European countries including Denmark and France and; ii) the spread of distinct clones dependent on local trading of pigs between farms. Phylogenetically related Italian and Danish LA-MRSA ST398 isolates shared extensive similarities including carriage of antimicrobial resistance genes. Our findings highlight the potential risk of transboundary transmission of antimicrobial-resistant bacterial clones with a high zoonotic potential when importing pigs from countries with high LA-MRSA prevalence. |
| Document Type: |
article in journal/newspaper |
| File Description: |
text |
| Language: |
English |
| ISSN: |
0099-2240 |
| Relation: |
https://researchonline.rvc.ac.uk/id/eprint/12699/1/12699_Local%20and%20transboundary%20transmission%20of%20methicillin-resistant%20Staphylococcus%20aureus%20sequence%20type%20398%20through%20pig%20trading_GREEN%20AAM.pdf; Pirolo, M and Sieber, R N and Moodley, A and Visaggio, D and Artuso, I and Gioffrè, A and Casalinuovo, F and Spatari, G and Guardabassi, L and Stegger, M and Visca, P (2020) Local and transboundary transmission of methicillin-resistant Staphylococcus aureus sequence type 398 through pig trading. Applied and Environmental Microbiology. ISSN 0099-2240 |
| Availability: |
https://researchonline.rvc.ac.uk/id/eprint/12699/; https://researchonline.rvc.ac.uk/id/eprint/12699/1/12699_Local%20and%20transboundary%20transmission%20of%20methicillin-resistant%20Staphylococcus%20aureus%20sequence%20type%20398%20through%20pig%20trading_GREEN%20AAM.pdf |
| Rights: |
cc_by_nc_4 |
| Accession Number: |
edsbas.7AB16412 |
| Database: |
BASE |