| Title: |
High-frequency neuronal bursting is essential for circadian and sleep behaviors in drosophila |
| Authors: |
Fernández, Florencia; Frenkel, Lia; Colque, Carina Celeste; Ricciuti, Ana; Hahm, Bryan; Cerredo, Karina; Muraro, Nara Ines; Ceriani, Maria Fernanda |
| Publisher Information: |
Society for Neuroscience |
| Collection: |
CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) |
| Subject Terms: |
BURSTING NEURON; DROSOPHILA MELANOGASTER; HCN; IH; ION CHANNEL; PIGMENT DISPERSING FACTOR; https://purl.org/becyt/ford/1.6; https://purl.org/becyt/ford/1 |
| Description: |
Circadian rhythms have been extensively studied in Drosophila; however, still little is known about how the electrical properties of clock neurons are specified. We have performed a behavioral genetic screen through the downregulation of candidate ion channels in the lateral ventral neurons (LNvs) and show that the hyperpolarization-activated cation current Ih is important for the behaviors that the LNvs influence: temporal organization of locomotor activity, analyzed in males, and sleep, analyzed in females. Using whole-cell patch clamp electrophysiology we demonstrate that small LNvs (sLNvs) are bursting neurons, and that Ih is necessary to achieve the high-frequency bursting firing pattern characteristic of both types of LNvs in females. Since firing in bursts has been associated to neuropeptide release, we hypothesized that Ih would be important for LNvs communication. Indeed, herein we demonstrate that Ih is fundamental for the recruitment of pigment dispersing factor (PDF) filled dense core vesicles (DCVs) to the terminals at the dorsal protocerebrum and for their timed release, and hence for the temporal coordination of circadian behaviors. ; Fil: Fernández, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina ; Fil: Frenkel, Lia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biociencias, Biotecnología y Biología Traslacional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina ; Fil: Colque, Carina Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones ... |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/url/https://www.jneurosci.org/content/41/4/689; https://hdl.handle.net/11336/143752; CONICET Digital; CONICET |
| Availability: |
https://hdl.handle.net/11336/143752 |
| Rights: |
info:eu-repo/semantics/openAccess ; https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
| Accession Number: |
edsbas.7C970657 |
| Database: |
BASE |