| Title: |
Bacillus safensis APC 4099 has broad-spectrum antimicrobial activity against both bacteria and fungi and produces several antimicrobial peptides, including the novel circular bacteriocin safencin E |
| Authors: |
Kamilari, Eleni; O’Connor, Paula M.; de Farias, Felipe Miceli; Johnson, C. N.; Buttimer, Colin; Deliephan, Aiswariya; Hill, Daragh; Fursenko, Oxana; Wiese, Jonathan; Stanton, Catherine; Hill, Colin; Ross, R. Paul |
| Publisher Information: |
American Society for Microbiology |
| Publication Year: |
2025 |
| Collection: |
University College Cork, Ireland: Cork Open Research Archive (CORA) |
| Subject Terms: |
Bacillus safensis; Antimicrobial peptides; Circular bacteriocin; Safencin E; Pumilarin; Plantazolicin; Pumilacidin; Bacilysin; Bacillibactin; Food biopreservation |
| Description: |
Bacillus safensis APC 4099, isolated from bees’ gut, has been identified as a promising candidate for food biopreservation. Antimicrobial activity screening revealed a broad-spectrum inhibition potential, ranging from gram-positive pathogenic bacteria to fungi responsible for food spoilage. Genomic analysis identified biosynthetic gene clusters coding for several antimicrobial peptides and secondary metabolites. Specifically, a novel, anionic, 6 kDa circular bacteriocin, named safencin E, was detected, showing 52.5% similarity to butyrivibriocin AR10. Additionally, gene clusters coding for the biosynthesis of bacteriocins such as pumilarin and plantazolicin and biosynthetic pathways for secondary metabolites, including pumilacidin A, bacilysin, and bacillibactin, were identified. Matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis detected molecular masses correlating to safencin E, plantazolicin, pumilarin, and pumilacidin A from the cell-free supernatant, cell extracts, or both. Overall, the broad-spectrum antimicrobial activity of B. safensis APC 4099 indicates that this strain is a promising candidate for the biological control of food ecosystems and thus has the potential to enhance food safety. |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf; application/vnd.openxmlformats-officedocument.wordprocessingml.document |
| Language: |
English |
| Relation: |
info:eu-repo/grantAgreement/SFI/Research Centres Programme/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/; 20; Applied and Environmental Microbiology; https://hdl.handle.net/10468/17809; 91 |
| DOI: |
10.1128/aem.01942-24 |
| Availability: |
https://hdl.handle.net/10468/17809; https://doi.org/10.1128/aem.01942-24 |
| Rights: |
© 2024, Kamilari et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International license. ; https://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.7CC7179B |
| Database: |
BASE |