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Microbial metabolic pathways guide response to immune checkpoint blockade therapy

Title: Microbial metabolic pathways guide response to immune checkpoint blockade therapy
Authors: Mimpen,Iris L; Battaglia,Thomas W; Parra Martinez,Miguel; Toner-Bartelds, Catherine; Zeverijn,Laurien J; Geurts, Birgit S; Verkerk,Karlijn; Hoes,Louisa R; van Renterghem,Allard W J; Noe,Michael; Hofland,Ingrid; Broeks,Annegien; van der Noort,Vincent; Stigter, Edwin C A; Gulersonmez, Can M C; Burgering, Boudewijn M T; van Gogh, Merel; de Zoete, Marcel R; Gelderblom,Hans; Dijkstra, Krijn K; Wessels,Lodewyk F A; Voest, Emile E; CMM Groep Burgering; Cancer; CMM Sectie Molecular Cancer Research; MMB-mcb; Infection & Immunity; MS Medische Oncologie
Publication Year: 2026
Subject Terms: Taverne; Journal Article
Description: Studies have identified a link between specific microbiome-derived bacteria and immune checkpoint blockade (ICB) efficacy. However, these species lack consistency across studies, and their immunomodulatory mechanisms remain elusive. To understand the influence of the microbiome on ICB response, we studied its functional capacity. Using pan-cancer metagenomics data from ICB-treated patients, we showed that community-level metabolic pathways are stable across individuals, making them suitable for predicting ICB response. We identified several microbial metabolic processes significantly associated with response, including the methylerythritol 4-phosphate (MEP) pathway, which was associated with response and induced Vδ2 T cell–mediated antitumor responses in patient-derived tumor organoids. In contrast, riboflavin synthesis was associated with ICB resistance, and its intermediates induced mucosal-associated invariant T (MAIT) cell–mediated immune suppression. Moreover, gut metabolomics revealed that high riboflavin levels were linked to worse survival in patients with abundant intratumoral MAIT cells. Collectively, our results highlight the relevance of metabolite-mediated microbiome–immune cell cross-talk.
Document Type: article in journal/newspaper
File Description: application/pdf
Language: English
ISSN: 2159-8274
Relation: https://dspace.library.uu.nl/handle/1874/468355
Availability: https://dspace.library.uu.nl/handle/1874/468355
Rights: info:eu-repo/semantics/EmbargoedAccess
Accession Number: edsbas.7CE60FBE
Database: BASE