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Metabolic deficiencies underlie reduced plasmacytoid dendritic cell IFN-I production following viral infection

Title: Metabolic deficiencies underlie reduced plasmacytoid dendritic cell IFN-I production following viral infection
Authors: Trever T. Greene; Yeara Jo; Carolina Chiale; Monica Macal; Ziyan Fang; Fawziyah S. Khatri; Alicia L. Codrington; Katelynn R. Kazane; Elizabeth Akbulut; Shobha Swaminathan; Yu Fujita; Patricia Fitzgerald-Bocarsly; Thekla Cordes; Christian Metallo; David A. Scott; Elina I. Zúñiga
Source: Nature Communications, Vol 16, Iss 1, Pp 1-19 (2025)
Publisher Information: Nature Portfolio
Publication Year: 2025
Collection: Directory of Open Access Journals: DOAJ Articles
Subject Terms: Science
Description: Type I Interferons (IFN-I) are central to host protection against viral infections, with plasmacytoid dendritic cells (pDC) being the most significant source, yet pDCs lose their IFN-I production capacity following an initial burst of IFN-I, resulting in susceptibility to secondary infections. The underlying mechanisms of these dynamics are not well understood. Here we find that viral infection reduces the capacity of pDCs to engage both oxidative and glycolytic metabolism. Mechanistically, we identify lactate dehydrogenase B (LDHB) as a positive regulator of pDC IFN-I production in mice and humans; meanwhile, LDHB deficiency is associated with suppressed IFN-I production, pDC metabolic capacity, and viral control following infection. In addition, preservation of LDHB expression is sufficient to partially retain the function of otherwise exhausted pDCs, both in vitro and in vivo. Furthermore, restoring LDHB in vivo in pDCs from infected mice increases IFNAR-dependent, infection-associated pathology. Our work thus identifies a mechanism for balancing immunity and pathology during viral infections, while also providing insight into the highly preserved infection-driven pDC inhibition.
Document Type: article in journal/newspaper
Language: English
Relation: https://doi.org/10.1038/s41467-025-56603-5; https://doaj.org/toc/2041-1723; https://doaj.org/article/5b08216b5e0049c599210f9c2d60e07b
DOI: 10.1038/s41467-025-56603-5
Availability: https://doi.org/10.1038/s41467-025-56603-5; https://doaj.org/article/5b08216b5e0049c599210f9c2d60e07b
Accession Number: edsbas.7D4CDBB1
Database: BASE