| Title: |
CBF4 is a unique member of the CBF transcription factor family of Vitis vinifera and Vitis riparia |
| Authors: |
XIAO, HUOGEN; TATTERSALL, ELIZABETH A. R.; SIDDIQUA, MAHBUBA K.; CRAMER, GRANT R.; NASSUTH, ANNETTE |
| Source: |
Plant, Cell & Environment ; volume 31, issue 1, page 1-10 ; ISSN 0140-7791 1365-3040 |
| Publisher Information: |
Wiley |
| Publication Year: |
2007 |
| Collection: |
Wiley Online Library (Open Access Articles via Crossref) |
| Description: |
The CBF/DREB1 transcription factors control an important pathway for increased freezing and drought tolerance in plants. We report here the isolation of one CBF/DREB1 ‐like gene, CBF4 , from both freezing‐tolerant wild grape ( Vitis riparia ) and freezing‐sensitive cultivated grape ( Vitis vinifera ). The deduced protein in V. riparia is 99% identical to the corresponding protein in V. vinifera ; 45–48% to three other Vitis CBF proteins reported earlier and 57% to AtCBF1, and contains CBF‐specific amino acid motifs. Agroinfiltration experiments in tobacco leaves revealed that VrCBF4 activates expression from reporter genes driven by a CRT‐containing promoter. Expression of the endogenous Vitis CBF4 genes was low at ambient temperature, but enhanced upon exposure to low temperature (4 °C). Uncommon for CBF genes, this expression was maintained for several days. No significant difference in expression pattern was observed between V. riparia and V. vinifera . Vitis CBF4 was expressed in both young and mature tissue, in contrast to the previously described Vitis CBF1, 2 and 3 . Together, these results suggest that CBF4 represents a second type of CBF in grape that might be more important for the over‐wintering of grape plants. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1111/j.1365-3040.2007.01741.x |
| Availability: |
https://doi.org/10.1111/j.1365-3040.2007.01741.x; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-3040.2007.01741.x; https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-3040.2007.01741.x |
| Rights: |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
| Accession Number: |
edsbas.7DBD526E |
| Database: |
BASE |