| Title: |
The antirepressor of phage P1 Isolation and interaction with the C1 repressor of P1 and P7 |
| Authors: |
Riedel, Hans-Dieter; Heinrich, Jochen; Heisig, Anke; Choli, Theodora; Schuster, Heinz |
| Source: |
FEBS Letters ; volume 334, issue 2, page 165-169 ; ISSN 0014-5793 1873-3468 |
| Publisher Information: |
Wiley |
| Publication Year: |
1993 |
| Collection: |
Wiley Online Library (Open Access Articles via Crossref) |
| Description: |
Two antirepressor proteins, Ant1 and Ant2, of molecular weight 42 and 32 kDa, respectively, are encoded by P1 as a single open reading frame, with the smaller protein initiating at an in‐frame start codon. Another open reading frame, icd , 5′ upstream of and overlapping ant 1 is required for ant 1 expression. Using appropriate ant gene‐carrying plasmids we have overproduced and purified Ant½ in the form of a protein complex and Ant2 as a single protein. Sequence analysis confirmed the N‐terminal amino acids predicted from the DNA sequence of ant 1/ ant 2, except that the N‐terminal methionine is missing in the Ant2 protein. Under appropriate conditions the C1 repressors of phages P1 and P7 specifically co‐precipitate with the Ant½ complex but not with Ant2 protein alone. The results suggest that the antirepressor may exert its C1‐inactivating function by a direct protein—protein interaction. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1016/0014-5793(93)81705-5 |
| DOI: |
10.1016/0014-5793%2893%2981705-5 |
| Availability: |
https://doi.org/10.1016/0014-5793(93)81705-5; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2F0014-5793%2893%2981705-5; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2F0014-5793(93)81705-5; https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/0014-5793%2893%2981705-5 |
| Rights: |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
| Accession Number: |
edsbas.7DF3E22D |
| Database: |
BASE |