| Title: |
Proteome-Wide Zika Virus CD4 T Cell Epitope and HLA Restriction Determination |
| Authors: |
Campbell, Victoria L; Nguyen, LeAnn; Snoey, Elise; McClurkan, Christopher L; Laing, Kerry J; Dong, Lichun; Sette, Alessandro; Lindestam Arlehamn, Cecilia S; Altmann, Danny M; Boyton, Rosemary J; Roby, Justin A; Gale, Michael; Stone, Mars; Busch, Michael P; Norris, Phillip J; Koelle, David M |
| Source: |
ImmunoHorizons, vol 4, iss 8 |
| Publisher Information: |
eScholarship, University of California |
| Publication Year: |
2020 |
| Collection: |
University of California: eScholarship |
| Subject Terms: |
3207 Medical Microbiology (for-2020); 32 Biomedical and Clinical Sciences (for-2020); 3204 Immunology (for-2020); Vaccine Related (rcdc); Biodefense (rcdc); Emerging Infectious Diseases (rcdc); Immunization (rcdc); Infectious Diseases (rcdc); Rare Diseases (rcdc); Vector-Borne Diseases (rcdc); Infection (hrcs-hc); 3 Good Health and Well Being (sdg); Adult (mesh); Aged (mesh); Animals (mesh); CD4-Positive T-Lymphocytes (mesh); Chlorocebus aethiops (mesh); Cross Reactions (mesh); Epitopes; T-Lymphocyte (mesh); Female (mesh); Humans (mesh); Male (mesh); Middle Aged (mesh); Proteome (mesh); Vero Cells (mesh); Young Adult (mesh); Zika Virus (mesh); Zika Virus Infection (mesh) |
| Subject Geographic: |
444 - 453 |
| Description: |
Zika virus (ZIKV) is a mosquito-borne pathogen that caused an epidemic in 2015-2016. ZIKV-specific T cell responses are functional in animal infection models, and helper CD4 T cells promote avid Abs in the vaccine context. The small volumes of blood available from field research limit the determination of T cell epitopes for complex microbes such as ZIKV. The goal of this project was efficient determination of human ZIKV CD4 T cell epitopes at the whole proteome scale, including validation of reactivity to whole pathogen, using small blood samples from convalescent time points when T cell response magnitude may have waned. Polyclonal enrichment of candidate ZIKV-specific CD4 T cells used cell-associated virus, documenting that T cells in downstream peptide analyses also recognize whole virus after Ag processing. Sequential query of bulk ZIKV-reactive CD4 T cells with pooled/single ZIKV peptides and molecularly defined APC allowed precision epitope and HLA restriction assignments across the ZIKV proteome and enabled discovery of numerous novel ZIKV CD4 T cell epitopes. The research workflow is useful for the study of emerging infectious diseases with a very limited human blood sample availability. |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
unknown |
| Relation: |
qt4dt0h3nw; https://escholarship.org/uc/item/4dt0h3nw; https://escholarship.org/content/qt4dt0h3nw/qt4dt0h3nw.pdf |
| DOI: |
10.4049/immunohorizons.2000068 |
| Availability: |
https://escholarship.org/uc/item/4dt0h3nw; https://escholarship.org/content/qt4dt0h3nw/qt4dt0h3nw.pdf; https://doi.org/10.4049/immunohorizons.2000068 |
| Rights: |
public |
| Accession Number: |
edsbas.80BA8250 |
| Database: |
BASE |