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Post-transcriptional Gene Regulation by MicroRNA-194 Promotes Neuroendocrine Transdifferentiation in Prostate Cancer

Title: Post-transcriptional Gene Regulation by MicroRNA-194 Promotes Neuroendocrine Transdifferentiation in Prostate Cancer
Authors: Fernandes, RC; Toubia, J; Townley, S; Hanson, AR; Dredge, BK; Pillman, KA; Bert, AG; Winter, JM; Iggo, R; Das, R; Obinata, D; Sandhu, S; Risbridger, GP; Taylor, RA; Lawrence, MG; Butler, LM; Zoubeidi, A; Gregory, PA; Tilley, WD; Hickey, TE; Goodall, GJ; Selth, LA
Publisher Information: CELL PRESS
Publication Year: 2021
Collection: The University of Melbourne: Digital Repository
Description: Potent therapeutic inhibition of the androgen receptor (AR) in prostate adenocarcinoma can lead to the emergence of neuroendocrine prostate cancer (NEPC), a phenomenon associated with enhanced cell plasticity. Here, we show that microRNA-194 (miR-194) is a regulator of epithelial-neuroendocrine transdifferentiation. In clinical prostate cancer samples, miR-194 expression and activity were elevated in NEPC and inversely correlated with AR signaling. miR-194 facilitated the emergence of neuroendocrine features in prostate cancer cells, a process mediated by its ability to directly target a suite of genes involved in cell plasticity. One such target was FOXA1, which encodes a transcription factor with a vital role in maintaining the prostate epithelial lineage. Importantly, a miR-194 inhibitor blocked epithelial-neuroendocrine transdifferentiation and inhibited the growth of cell lines and patient-derived organoids possessing neuroendocrine features. Overall, our study reveals a post-transcriptional mechanism regulating the plasticity of prostate cancer cells and provides a rationale for targeting miR-194 in NEPC.
Document Type: article in journal/newspaper
Language: English
ISSN: 2211-1247
Relation: https://hdl.handle.net/11343/280222
Availability: https://hdl.handle.net/11343/280222
Rights: https://creativecommons.org/licenses/by-nc-nd/4.0 ; CC BY-NC-ND
Accession Number: edsbas.81AC9B48
Database: BASE