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Human CRY1 variants associate with attention deficit/hyperactivity disorder

Title: Human CRY1 variants associate with attention deficit/hyperactivity disorder
Authors: Emre Onat O.; Ece Kars M.; Gul S.; Bilguvar K.; Wu Y.; Ozhan A.; Aydin C.; Nazli Basak A.; Allegra Trusso M.; Goracci A.; Fallerini C.; Renieri A.; Casanova J. -L.; Itan Y.; Atbasoglu C. E.; Saka M. C.; Halil Kavakli I.; Ozcelik T.
Contributors: Emre Onat, O.; Ece Kars, M.; Gul, S.; Bilguvar, K.; Wu, Y.; Ozhan, A.; Aydin, C.; Nazli Basak, A.; Allegra Trusso, M.; Goracci, A.; Fallerini, C.; Renieri, A.; Casanova, J. -L.; Itan, Y.; Atbasoglu, C. E.; Saka, M. C.; Halil Kavakli, I.; Ozcelik, T.
Publication Year: 2020
Collection: Università degli Studi di Siena: USiena air
Subject Terms: Genetic diseases; Genetics; Monogenic diseases; Psychiatric diseases; ARNTL Transcription Factors; Adult; Attention Deficit Disorder with Hyperactivity; CLOCK Proteins; Cryptochromes; Female; Genetic Association Studies; HEK293 Cells; Humans; Male; Sleep Disorders; Circadian Rhythm; Mutation
Description: Attention deficit/hyperactivity disorder (ADHD) is a common and heritable phenotype frequently accompanied by insomnia, anxiety, and depression. Here, using a reverse phenotyping approach, we report heterozygous coding variations in the core circadian clock gene cryptochrome 1 in 15 unrelated multigenerational families with combined ADHD and insomnia. The variants led to functional alterations in the circadian molecular rhythms, providing a mechanistic link to the behavioral symptoms. One variant, CRY1Δ11 c.1657+3A>C, is present in approximately 1% of Europeans, therefore standing out as a diagnostic and therapeutic marker. We showed by exome sequencing in an independent cohort of patients with combined ADHD and insomnia that 8 of 62 patients and 0 of 369 controls carried CRY1Δ11. Also, we identified a variant, CRY1Δ6 c.825+1G>A, that shows reduced affinity for BMAL1/CLOCK and causes an arrhythmic phenotype. Genotype-phenotype correlation analysis revealed that this variant segregated with ADHD and delayed sleep phase disorder (DSPD) in the affected family. Finally, we found in a phenome-wide association study involving 9438 unrelated adult Europeans that CRY1Δ11 was associated with major depressive disorder, insomnia, and anxiety. These results defined a distinctive group of circadian psychiatric phenotypes that we propose to designate as “circiatric” disorders.
Document Type: article in journal/newspaper
File Description: STAMPA
Language: English
Relation: info:eu-repo/semantics/altIdentifier/pmid/32538895; info:eu-repo/semantics/altIdentifier/wos/WOS:000579359700002; volume:130; issue:7; firstpage:3885; lastpage:3900; numberofpages:16; journal:THE JOURNAL OF CLINICAL INVESTIGATION; https://hdl.handle.net/11365/1133646; https://www.jci.org/articles/view/135500; https://pmc.ncbi.nlm.nih.gov/articles/PMC7324179/
DOI: 10.1172/JCI135500
Availability: https://hdl.handle.net/11365/1133646; https://doi.org/10.1172/JCI135500; https://www.jci.org/articles/view/135500; https://pmc.ncbi.nlm.nih.gov/articles/PMC7324179/
Rights: info:eu-repo/semantics/closedAccess
Accession Number: edsbas.81D3EB01
Database: BASE